The parallel flow drop solvothermal method was utilized to synthesize the crystal of MOF-5 by taking the molar ratio of the metal ions to the organic ligands of 2:1 at 140C, and the reaction time at 12 hours. Meanwhile, the structure and properties of MOF-5 were characterized by the X-ray diffraction (XRD), scanning electron microscope (SEM), thermogravimetric analysis (TGA) and fourier transform infrared spectroscopy (FTIR). SEM analysis shown that the crystal morphology of MOF-5 changed from sheet to cubic with increasing reaction temperature and molar ratio of the metal ions to the organic ligands, and its thermal stability was also gradually increased. TGA analysis shown that its thermal stability could live up to 489.36C. FTIR analysis shown that the terephthalic acid is completely protonated, and the Zn2+ and the carboxyl group are formed by the coordination of the multi-tooth bridge in the crystal of MOF-5. Then the structure activity relationship of the crystal MOF-5 synthesis, microstructural, thermal stability and N2 adsorption property were further studied. © 2020 S. Wang et al., published by De Gruyter 2020.