Preparative separation and purification of Rebaudioside A from Stevia rebaudiana Bertoni crude extracts by mixed bed of macroporous adsorption resins

doi:10.1016/j.foodchem.2011.10.077

	Preparative separation and purification of Rebaudioside A from Stevia rebaudiana Bertoni crude extracts by mixed bed of macroporous adsorption resins
	Li, Jie 1,2; Chen, Zhenbin 1,2,3; Di, Duolong 3
刊名	FOOD CHEMISTRY
	2012-05
卷号	132 期号:1 页码:268-276
关键词	Separation Rebaudioside A Stevia Rebaudiana Bertoni Steviosides Macroporous adsorption resin mixed bed
ISSN号	0308-8146
DOI	10.1016/j.foodchem.2011.10.077
英文摘要	The separation and purification of Rebaudioside A (RA) from Stevia Rebaudiana Bertoni crude extracts (Steviosides) by macroporous adsorption resin (MAR) mixed bed were systematically investigated. MAR mixed bed of HPD750-LSA40-LSA30-DS401 was selected due to its better separation degree. Based on the kinetics/thermodynamics experiment of the mixed bed, it was found that the experimental data fitted better to the pseudo-second-order model, and intra-particle diffusion was rate-limiting step. The adsorption isotherm was consistent with IV equilibrium adsorption isotherm classified by Brunauer. Furthermore, the influencing factors for the separation of RA based on HPLC were also investigated. Under the optimal conditions, the separation degree for RA (D-AS) increased from 0.771 to 1.54. Moreover, the experimental results showed that the purity of the obtained product increased from 60% to 97%. (C) 2011 Elsevier Ltd. All rights reserved.
资助项目	Gansu Provincial Department of Education[1001ZSB002]
WOS研究方向	Chemistry ; Food Science & Technology ; Nutrition & Dietetics
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000300465100035
状态	已发表
内容类型	期刊论文
源URL	[http://119.78.100.223/handle/2XXMBERH/34912]
专题	材料科学与工程学院省部共建有色金属先进加工与再利用国家重点实验室
通讯作者	Chen, Zhenbin
作者单位	1.Lanzhou Univ Technol, State Key Lab Gansu Adv Nonferrous Met Mat, Lanzhou 730050, Gansu, Peoples R China 2.Lanzhou Univ Technol, Sch Mat Sci & Engn, Lanzhou 730050, Gansu, Peoples R China 3.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Li, Jie,Chen, Zhenbin,Di, Duolong. Preparative separation and purification of Rebaudioside A from Stevia rebaudiana Bertoni crude extracts by mixed bed of macroporous adsorption resins[J]. FOOD CHEMISTRY,2012,132(1):268-276.
APA	Li, Jie,Chen, Zhenbin,&Di, Duolong.(2012).Preparative separation and purification of Rebaudioside A from Stevia rebaudiana Bertoni crude extracts by mixed bed of macroporous adsorption resins.FOOD CHEMISTRY,132(1),268-276.
MLA	Li, Jie,et al."Preparative separation and purification of Rebaudioside A from Stevia rebaudiana Bertoni crude extracts by mixed bed of macroporous adsorption resins".FOOD CHEMISTRY 132.1(2012):268-276.