Refolding of a recombinant full-length non-structural (NS3) protein from hepatitis C virus by chromatographic procedures

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	Refolding of a recombinant full-length non-structural (NS3) protein from hepatitis C virus by chromatographic procedures
	Li, M ; Poliakov, A ; Danielson, UH ; Su, ZG ; Janson, JC
刊名	BIOTECHNOLOGY LETTERS
	2003-10-01
卷号	25 期号:20 页码:1729-1734
关键词	gel filtration HCV immobilized metal affinity chromatography ion-exchange chromatography NS3 refolding
ISSN号	0141-5492
其他题名	Biotechnol. Lett.
中文摘要	The non-structural protein 3 (NS3) of Hepatitis C virus (HCV) was expressed as inactive aggregates in Escherichia coli. The protein was refolded by chromatographic techniques of which ion exchange chromatography was best for crude samples and gel filtration best for partially purified samples. Immobilized metal ion affinity chromatography showed intermediate performance. Gradient procedures enhanced the recovery of active NS3 protein.
英文摘要	The non-structural protein 3 (NS3) of Hepatitis C virus (HCV) was expressed as inactive aggregates in Escherichia coli. The protein was refolded by chromatographic techniques of which ion exchange chromatography was best for crude samples and gel filtration best for partially purified samples. Immobilized metal ion affinity chromatography showed intermediate performance. Gradient procedures enhanced the recovery of active NS3 protein.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	ION-EXCHANGE CHROMATOGRAPHY ; PURIFICATION
收录类别	SCI
原文出处	://WOS:000185682000008
语种	英语
WOS记录号	WOS:000185682000008
公开日期	2013-11-08
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/5343]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Uppsala Univ, Dept Surface Biotechnol, BMC, SE-75123 Uppsala, Sweden 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100864, Peoples R China 3.Uppsala Univ, Dept Biochem, BMC, SE-75123 Uppsala, Sweden
推荐引用方式 GB/T 7714	Li, M,Poliakov, A,Danielson, UH,et al. Refolding of a recombinant full-length non-structural (NS3) protein from hepatitis C virus by chromatographic procedures[J]. BIOTECHNOLOGY LETTERS,2003,25(20):1729-1734.
APA	Li, M,Poliakov, A,Danielson, UH,Su, ZG,&Janson, JC.(2003).Refolding of a recombinant full-length non-structural (NS3) protein from hepatitis C virus by chromatographic procedures.BIOTECHNOLOGY LETTERS,25(20),1729-1734.
MLA	Li, M,et al."Refolding of a recombinant full-length non-structural (NS3) protein from hepatitis C virus by chromatographic procedures".BIOTECHNOLOGY LETTERS 25.20(2003):1729-1734.