The preparation of an artificial red blood cell substitute by W/O/W double emulsion methods

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	The preparation of an artificial red blood cell substitute by W/O/W double emulsion methods
	Qiu, W; Meng, FT; Ma, GH; Su, ZG
刊名	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
	2003-05-01
卷号	35 期号:5 页码:467-472
关键词	artificial red cells methoxypoly(ethylene glycol)-poly-DL-lactide bovine hemoglobin microcapsule
ISSN号	0582-9879
其他题名	Acta Biochim. Biophys. Sin.
中文摘要	A method of preparing methoxypoly (ethylene glycol)-poly-DL-lactide (PELA) microcapsule containing bovine hemoglobin(BHb) by using W/O/W double emulsion-solvent diffusion process for use as artificial red cells was developed. The results suggested that preparation conditions such as solidification method, stirring rate, type of organic solvent had significant influence on the encapsulation efficiency and the activity of bovine hemoglobin. When ethyl acetate was used as the organic solvent and double emulsion-solvent diffusion process was used, there was no significant influence on the activity of hemoglobin if stirring rate was lower than 9000 r/min. High stirring rate, 12 000 r/min, decreased the P-50 and Hill coefficient of the hemoglobin encapsulated. Increasing the volume of solidification solution had an effect of improving the activity of hemoglobin to carry oxygen.
英文摘要	A method of preparing methoxypoly (ethylene glycol)-poly-DL-lactide (PELA) microcapsule containing bovine hemoglobin(BHb) by using W/O/W double emulsion-solvent diffusion process for use as artificial red cells was developed. The results suggested that preparation conditions such as solidification method, stirring rate, type of organic solvent had significant influence on the encapsulation efficiency and the activity of bovine hemoglobin. When ethyl acetate was used as the organic solvent and double emulsion-solvent diffusion process was used, there was no significant influence on the activity of hemoglobin if stirring rate was lower than 9000 r/min. High stirring rate, 12 000 r/min, decreased the P-50 and Hill coefficient of the hemoglobin encapsulated. Increasing the volume of solidification solution had an effect of improving the activity of hemoglobin to carry oxygen.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]	Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]	MEMBRANE HEMOGLOBIN NANOCAPSULES ; POLY(LACTIC ACID) ; MICROSPHERES ; PROTEIN ; ENCAPSULATION ; PEG
收录类别	SCI
原文出处	://WOS:000183076800012
语种	英语
WOS记录号	WOS:000183076800012
公开日期	2013-11-08
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/5332]
专题	过程工程研究所_研究所（批量导入）
作者单位	Chinese Acad Sci, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Qiu, W,Meng, FT,Ma, GH,et al. The preparation of an artificial red blood cell substitute by W/O/W double emulsion methods[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(5):467-472.
APA	Qiu, W,Meng, FT,Ma, GH,&Su, ZG.(2003).The preparation of an artificial red blood cell substitute by W/O/W double emulsion methods.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(5),467-472.
MLA	Qiu, W,et al."The preparation of an artificial red blood cell substitute by W/O/W double emulsion methods".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.5(2003):467-472.