Oligomannuronate prevents mitochondrial dysfunction induced by IAPP in RINm5F islet cells by inhibition of JNK activation and cell apoptosis

doi:10.1186/s13020-020-00310-4

CORC > 兰州理工大学 > 兰州理工大学

	Oligomannuronate prevents mitochondrial dysfunction induced by IAPP in RINm5F islet cells by inhibition of JNK activation and cell apoptosis
	Xi Liu; Qiong Li; Xiaolei Cheng; Zhichun Liu; Xiaoliang Zhao; Guangli Yu; Jiejie Hao
刊名	Chinese Medicine
	2020
卷号	15 期号:1 页码:1-12.
关键词	Oligomannuronate Type 2 diabetes Islet amyloid polypeptide JNK activation Mitochondrial dysfunction RINm5F cells
ISSN号	1749-8546
DOI	10.1186/s13020-020-00310-4
英文摘要	Background Oligomannuronates (OM) are natural products from alginate that is frequently used as food supplement. The aim of this study was to investigate the in vitro protective effects of OM on RINm5F cells against human Islet amyloid polypeptide (IAPP) induced mitochondrial dysfunction, as well as the underlying mechanisms. Methods In the present study, we obtained several kinds of OM with different molecular masses, and then we used RINm5F cells as a model to elucidate the involvement of JNK signal pathway in hIAPP-induced mitochondrial dysfunction in pancreatic beta cells, and the protective effects of OM are associated with its ability to attenuate the mitochondrial dysfunction. Results Our results demonstrated that human IAPP induced mitochondrial dysfunction, as evidence by loss of Delta psi m and ATP content, and decrease in oxygen consumption and complex activities, was accompanied by JNK activation, changes in the expressions of Bcl-2 and Bax proteins, release of cytochrome c (Cyto-c) and apoptosis inducing factor (AIF) from mitochondria into cytosol. Interestingly, the human IAPP induced damage in RINm5F cells were effectively restored by co-treatment of OM. Moreover, JNK activation was required for the OM mediated changes in RINm5F cells. Conclusions OM prevented mitochondrial dysfunction induced by human IAPP in RINm5F islet cells through JNK dependent signaling pathways.
WOS研究方向	Integrative & Complementary Medicine ; Pharmacology & Pharmacy
语种	英语
出版者	BMC
WOS记录号	WOS:000522125200001
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/156359]
专题	兰州理工大学
通讯作者	Jiejie Hao
推荐引用方式 GB/T 7714	Xi Liu,Qiong Li,Xiaolei Cheng,et al. Oligomannuronate prevents mitochondrial dysfunction induced by IAPP in RINm5F islet cells by inhibition of JNK activation and cell apoptosis[J]. Chinese Medicine,2020,15(1):1-12..
APA	Xi Liu.,Qiong Li.,Xiaolei Cheng.,Zhichun Liu.,Xiaoliang Zhao.,...&Jiejie Hao.(2020).Oligomannuronate prevents mitochondrial dysfunction induced by IAPP in RINm5F islet cells by inhibition of JNK activation and cell apoptosis.Chinese Medicine,15(1),1-12..
MLA	Xi Liu,et al."Oligomannuronate prevents mitochondrial dysfunction induced by IAPP in RINm5F islet cells by inhibition of JNK activation and cell apoptosis".Chinese Medicine 15.1(2020):1-12..