In vitro protein refolding by chromatographic procedures

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	In vitro protein refolding by chromatographic procedures
	Li, M ; Su, ZG ; Janson, JC
刊名	PROTEIN EXPRESSION AND PURIFICATION
	2004
卷号	33 期号:1 页码:1-10
关键词	in vitro protein refolding inclusion bodies chromatographic techniques gradient techniques
ISSN号	1046-5928
其他题名	Protein Expr. Purif.
中文摘要	In vitro protein refolding is still a bottleneck in both structural biology and in the development of new biopharmaceuticals, especially for commercially important polypeptides that are overexpressed in Escherichia coli. This review focuses on protein refolding methods based on column procedures because recent advances in chromatographic refolding have shown promising results. (C) 2003 Elsevier Inc. All rights reserved.
英文摘要	In vitro protein refolding is still a bottleneck in both structural biology and in the development of new biopharmaceuticals, especially for commercially important polypeptides that are overexpressed in Escherichia coli. This review focuses on protein refolding methods based on column procedures because recent advances in chromatographic refolding have shown promising results. (C) 2003 Elsevier Inc. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
研究领域[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
关键词[WOS]	SIZE-EXCLUSION CHROMATOGRAPHY ; ION-EXCHANGE CHROMATOGRAPHY ; IMMOBILIZED LIPOSOME CHROMATOGRAPHY ; UROKINASE-PLASMINOGEN-ACTIVATOR ; COLI INCLUSION-BODIES ; EGG-WHITE LYSOZYME ; ESCHERICHIA-COLI ; RECOMBINANT PROTEINS ; CARBONIC-ANHYDRASE ; STEP PURIFICATION
收录类别	SCI
原文出处	://WOS:000187587500001
语种	英语
WOS记录号	WOS:000187587500001
公开日期	2013-11-05
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/4952]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Uppsala Biomed Ctr, Dept Surface Biotechnol, SE-75123 Uppsala, Sweden 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China 3.Amersham Biosci R&D Dept, Uppsala, Sweden
推荐引用方式 GB/T 7714	Li, M,Su, ZG,Janson, JC. In vitro protein refolding by chromatographic procedures[J]. PROTEIN EXPRESSION AND PURIFICATION,2004,33(1):1-10.
APA	Li, M,Su, ZG,&Janson, JC.(2004).In vitro protein refolding by chromatographic procedures.PROTEIN EXPRESSION AND PURIFICATION,33(1),1-10.
MLA	Li, M,et al."In vitro protein refolding by chromatographic procedures".PROTEIN EXPRESSION AND PURIFICATION 33.1(2004):1-10.