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	Polystyrene-type uniform porous microsphere enables high resolution and low-pressure chromatography of natural products - A case study with icariin purification
	Sun, HH; Li, XN; Ma, GH; Su, ZG
刊名	CHROMATOGRAPHIA
	2005
卷号	61期号:1-2页码:9-15
关键词	column liquid chromatography icariin membrane emulsification uniform porous microsphere
ISSN号	0009-5893
其他题名	Chromatographia
中文摘要	An efficient low-pressure preparative chromatography for purification of icariin from crude extract has been developed by employing a novel uniform porous microsphere, named PST, as packing material. A comparison was made between the PST medium and a commercially available silica-based C-18 medium on their column chromatographic performance. The PST column demonstrated a higher resolution and better selectivity than the C-18 column. Crude extract of 20% icariin can be purified to 90.0% with the recovery of 99.9% and column loadability of 2.5 mg-mL(-1) medium on the PST column under the optimized condition. After crystallization the purity of icariin can reach more than 98% with a total recovery of 93%. The product structure was determined by means of UV, IR, MS and NMR.
英文摘要	An efficient low-pressure preparative chromatography for purification of icariin from crude extract has been developed by employing a novel uniform porous microsphere, named PST, as packing material. A comparison was made between the PST medium and a commercially available silica-based C-18 medium on their column chromatographic performance. The PST column demonstrated a higher resolution and better selectivity than the C-18 column. Crude extract of 20% icariin can be purified to 90.0% with the recovery of 99.9% and column loadability of 2.5 mg-mL(-1) medium on the PST column under the optimized condition. After crystallization the purity of icariin can reach more than 98% with a total recovery of 93%. The product structure was determined by means of UV, IR, MS and NMR.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]	ANTI-HYPOXIA/REOXYGENATION AGENTS ; LIQUID-CHROMATOGRAPHY ; FLAVONOL GLYCOSIDES ; RETENTION ; COLUMNS ; CELLS
收录类别	SCI
原文出处	://WOS:000226506000002
语种	英语
WOS记录号	WOS:000226506000002
公开日期	2013-10-25
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/4224]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Beijing Inst Chem Technol, Sch Chem Engn, Beijing 100029, Peoples R China
推荐引用方式 GB/T 7714	Sun, HH,Li, XN,Ma, GH,et al. Polystyrene-type uniform porous microsphere enables high resolution and low-pressure chromatography of natural products - A case study with icariin purification[J]. CHROMATOGRAPHIA,2005,61(1-2):9-15.
APA	Sun, HH,Li, XN,Ma, GH,&Su, ZG.(2005).Polystyrene-type uniform porous microsphere enables high resolution and low-pressure chromatography of natural products - A case study with icariin purification.CHROMATOGRAPHIA,61(1-2),9-15.
MLA	Sun, HH,et al."Polystyrene-type uniform porous microsphere enables high resolution and low-pressure chromatography of natural products - A case study with icariin purification".CHROMATOGRAPHIA 61.1-2(2005):9-15.

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