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	One-step purification of epigallocatechin gallate from crude green tea extracts by isocratic hydrogen bond adsorption chromatography on beta-cyclodextrin substituted agarose gel media
	Xu, J; Zhang, GF; Tan, TW; Janson, JC
刊名	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
	2005-09-25
卷号	824期号:1-2页码:323-326
关键词	beta-cyclodextrin tea polyphenols EGCG catechin
ISSN号	1570-0232
其他题名	J. Chromatogr. B
中文摘要	An oligomerized beta-cyclodextrin ligand coupled to brominated allyl-group substituted Sepharose HP has been used for the one-step purification of polyphenolic epigallocatechin gallate (EGCG), an important antioxidant, by isocratic hydrogen bond adsorption chromatography. With a sample load of 1.33 mg crude green tea polyphenolic extract per ml column packing and with water/ethanol/acetonitrile (57/30/13, v/v) as the optimum mobile phase, an EGCG purity of about 98% with a recovery of approximate 73% could be achieved by proper peak cutting. After about 10 sample applications, the column performance started to deteriorate but could be regenerated to its original function by cleaning with 0.35 M NaOH. (c) 2005 Elsevier B.V. All rights reserved.
英文摘要	An oligomerized beta-cyclodextrin ligand coupled to brominated allyl-group substituted Sepharose HP has been used for the one-step purification of polyphenolic epigallocatechin gallate (EGCG), an important antioxidant, by isocratic hydrogen bond adsorption chromatography. With a sample load of 1.33 mg crude green tea polyphenolic extract per ml column packing and with water/ethanol/acetonitrile (57/30/13, v/v) as the optimum mobile phase, an EGCG purity of about 98% with a recovery of approximate 73% could be achieved by proper peak cutting. After about 10 sample applications, the column performance started to deteriorate but could be regenerated to its original function by cleaning with 0.35 M NaOH. (c) 2005 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]	LIQUID-CHROMATOGRAPHY ; STATIONARY-PHASE ; CELLS ; SEPARATION
收录类别	SCI
原文出处	://WOS:000231768900041
语种	英语
WOS记录号	WOS:000231768900041
公开日期	2013-10-25
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/4213]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Uppsala Univ, Dept Surface Biotechnol, Uppsala Biomed Ctr, SE-75123 Uppsala, Sweden 2.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China 3.Beijing Univ Chem Technol, Coll Life Sci & Technol, Dept Bioengn, Beijing 100029, Peoples R China 4.Uppsala Univ, Dept Surface Biotechnol, Uppsala Biomed Ctr, SE-75123 Uppsala, Sweden 5.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China 6.Beijing Univ Chem Technol, Coll Life Sci & Technol, Dept Bioengn, Beijing 100029, Peoples R China
推荐引用方式 GB/T 7714	Xu, J,Zhang, GF,Tan, TW,et al. One-step purification of epigallocatechin gallate from crude green tea extracts by isocratic hydrogen bond adsorption chromatography on beta-cyclodextrin substituted agarose gel media[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2005,824(1-2):323-326.
APA	Xu, J,Zhang, GF,Tan, TW,&Janson, JC.(2005).One-step purification of epigallocatechin gallate from crude green tea extracts by isocratic hydrogen bond adsorption chromatography on beta-cyclodextrin substituted agarose gel media.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,824(1-2),323-326.
MLA	Xu, J,et al."One-step purification of epigallocatechin gallate from crude green tea extracts by isocratic hydrogen bond adsorption chromatography on beta-cyclodextrin substituted agarose gel media".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 824.1-2(2005):323-326.

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