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	Size characterization of green fluorescent protein inclusion bodies in E. coli using asymmetrical flow field-flow fractionation-multi-angle light scattering
	Luo, J; Leeman, M; Ballagi, A; Elfwing, A; Su, ZG; Janson, JC; Wahlund, KG
刊名	JOURNAL OF CHROMATOGRAPHY A
	2006-07-07
卷号	1120期号:1-2页码:158-164
关键词	asymmetrical flow field-flow fractionation multi-angle light scattering root mean square radius hydrodynamic diameter green fluorescence protein inclusion bodies
ISSN号	0021-9673
其他题名	J. Chromatogr. A
中文摘要	The goal of this study was to investigate the applicability of asymmetrical flow field-flow fractionation-multi-angle light scattering (AsFIFFFMALS) for size analysis of green fluorescent protein inclusion bodies (GFPIBs). The size distributions of GFPIBs prepared by various culture conditions were determined. For GFPIBs prepared at 37 degrees C the peak maximum hydrodynamic diameter (dH) first increased and then decreased with the increase of the induction times in the presence of 0.1 and 2 mM isopropyl-beta-D-thiogalactoside (IPTG). For GFPIBs prepared at 30 degrees C the peak maximum dH was constant at about 700 nm irrespectively of the induction times and IPTG concentrations. (c) 2005 Elsevier B.V. All rights reserved.
英文摘要	The goal of this study was to investigate the applicability of asymmetrical flow field-flow fractionation-multi-angle light scattering (AsFIFFFMALS) for size analysis of green fluorescent protein inclusion bodies (GFPIBs). The size distributions of GFPIBs prepared by various culture conditions were determined. For GFPIBs prepared at 37 degrees C the peak maximum hydrodynamic diameter (dH) first increased and then decreased with the increase of the induction times in the presence of 0.1 and 2 mM isopropyl-beta-D-thiogalactoside (IPTG). For GFPIBs prepared at 30 degrees C the peak maximum dH was constant at about 700 nm irrespectively of the induction times and IPTG concentrations. (c) 2005 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]	ESCHERICHIA-COLI ; MAMMALIAN-CELLS ; GENE-TRANSFER ; MOLAR-MASS ; RETENTION ; DISTRIBUTIONS ; POLYSTYRENE ; EXPRESSION ; SEPARATION ; DENSITY
收录类别	ISTP ; SCI
原文出处	://WOS:000238623400020
语种	英语
WOS记录号	WOS:000238623400020
公开日期	2013-10-24
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/4074]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Lund Univ, Fac Engn, Div Tech Analyt Chem, Ctr Chem & Chem Engn, SE-22100 Lund, Sweden 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 3.Uppsala Univ, Uppsala Biomed Ctr, Dept Surface Biotechnol, SE-75123 Uppsala, Sweden
推荐引用方式 GB/T 7714	Luo, J,Leeman, M,Ballagi, A,et al. Size characterization of green fluorescent protein inclusion bodies in E. coli using asymmetrical flow field-flow fractionation-multi-angle light scattering[J]. JOURNAL OF CHROMATOGRAPHY A,2006,1120(1-2):158-164.
APA	Luo, J.,Leeman, M.,Ballagi, A.,Elfwing, A.,Su, ZG.,...&Wahlund, KG.(2006).Size characterization of green fluorescent protein inclusion bodies in E. coli using asymmetrical flow field-flow fractionation-multi-angle light scattering.JOURNAL OF CHROMATOGRAPHY A,1120(1-2),158-164.
MLA	Luo, J,et al."Size characterization of green fluorescent protein inclusion bodies in E. coli using asymmetrical flow field-flow fractionation-multi-angle light scattering".JOURNAL OF CHROMATOGRAPHY A 1120.1-2(2006):158-164.

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