Purification of nattokinase by reverse micelles extraction from fermentation broth: effect of temperature and phase volume ratio

CORC > 过程工程研究所 > 中国科学院过程工程研究所 > 研究所（批量导入）

	Purification of nattokinase by reverse micelles extraction from fermentation broth: effect of temperature and phase volume ratio
	Liu, JG ; Xing, JM ; Chang, TS ; Liu, HZ
刊名	BIOPROCESS AND BIOSYSTEMS ENGINEERING
	2006-03-01
卷号	28 期号:4 页码:267-273
关键词	reverse micelles nattokinase AOT protein purification concentration
ISSN号	1615-7591
其他题名	Bioprocess. Biosyst. Eng.
中文摘要	Nattokinase is a novel fibrinolytic enzyme that is considered to be a promising agent for thrombosis therapy. In this study, reverse micelles extraction was applied to purify and concentrate nattokinase from fermentation broth. The effects of temperature and phase volume ratio used for the forward and backward extraction on the extraction process were examined. The optimal temperature for forward and backward extraction were 25 degrees C and 35 degrees C respectively. Nattokinase became more thermosensitive during reverse micelles extraction. And it could be enriched in the stripping phase eight times during backward extraction. It was found that nattokinase could be purified by AOT reverse micelles with up to 80% activity recovery and with a purification factor of 3.9.
英文摘要	Nattokinase is a novel fibrinolytic enzyme that is considered to be a promising agent for thrombosis therapy. In this study, reverse micelles extraction was applied to purify and concentrate nattokinase from fermentation broth. The effects of temperature and phase volume ratio used for the forward and backward extraction on the extraction process were examined. The optimal temperature for forward and backward extraction were 25 degrees C and 35 degrees C respectively. Nattokinase became more thermosensitive during reverse micelles extraction. And it could be enriched in the stripping phase eight times during backward extraction. It was found that nattokinase could be purified by AOT reverse micelles with up to 80% activity recovery and with a purification factor of 3.9.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]	Biotechnology & Applied Microbiology ; Engineering, Chemical
研究领域[WOS]	Biotechnology & Applied Microbiology ; Engineering
关键词[WOS]	FIBRINOLYTIC ENZYME NATTOKINASE ; VEGETABLE CHEESE NATTO ; BACK-EXTRACTION ; PARTITIONING BEHAVIOR ; LYSOZYME EXTRACTION ; RECOVERY ; SEPARATION ; MICROEMULSIONS ; ENHANCEMENT ; ENRICHMENT
收录类别	SCI
原文出处	://WOS:000235753900007
语种	英语
WOS记录号	WOS:000235753900007
公开日期	2013-10-24
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/4057]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Hebei Univ Sci & Technol, Coll Biosci & Bioengn, Shijiazhuang 050018, Hebei Province, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Lab Separat Sci & Engn, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Liu, JG,Xing, JM,Chang, TS,et al. Purification of nattokinase by reverse micelles extraction from fermentation broth: effect of temperature and phase volume ratio[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2006,28(4):267-273.
APA	Liu, JG,Xing, JM,Chang, TS,&Liu, HZ.(2006).Purification of nattokinase by reverse micelles extraction from fermentation broth: effect of temperature and phase volume ratio.BIOPROCESS AND BIOSYSTEMS ENGINEERING,28(4),267-273.
MLA	Liu, JG,et al."Purification of nattokinase by reverse micelles extraction from fermentation broth: effect of temperature and phase volume ratio".BIOPROCESS AND BIOSYSTEMS ENGINEERING 28.4(2006):267-273.