Partitioning behavior of penicillin G in aqueous two phase system formed by ionic liquids and phosphate

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	Partitioning behavior of penicillin G in aqueous two phase system formed by ionic liquids and phosphate
	Liu, Qingfen ; Yu, Jiang ; Li, Wangliang ; Hu, Xuesheng ; Xia, Hansong ; Liu, Huizhou ; Yang, Ping
刊名	SEPARATION SCIENCE AND TECHNOLOGY
	2006
卷号	41 期号:12 页码:2849-2858
关键词	ionic liquid extraction aqueous two-phase system penicillin emulsification
ISSN号	0149-6395
其他题名	Sep. Sci. Technol.
中文摘要	An aqueous two-phase system (ATPS) was presented with hydrophilic ionic liquid 1-butyl-3-methylimidazolium chloride ([Bmim]Cl) and NaH2PO4 aqueous solution in this paper. The partitioning behavior of penicillin G in the ATPS was investigated. Concentrations of NaH2PO4, penicillin G, and [Bmim]Cl were evaluated to determine their effects on the partition coefficient and extraction yield of penicillin G. It was found that both of partition coefficient and extraction yield strongly depended on the concentration of [Bmim]Cl, penicillin and NaH2PO4. A high extraction yield of 93% was achieved with the following parameters: NaH2PO4 center dot 2H(2)O 40% (wt%), penicillin 45000 similar to 50000 u/ml, [Bmim]Cl 20 similar to 21% (wt%). The [Bmim]Cl/NaH2PO4 system was also applied in a real filtration of penicillin G fermentation broth and the extraction yield was averaged at 91.5%. It is worthy noting that the working pH value of ATPS was at the range of 5 similar to 6, no emulsification and protein denaturation could be observed.
英文摘要	An aqueous two-phase system (ATPS) was presented with hydrophilic ionic liquid 1-butyl-3-methylimidazolium chloride ([Bmim]Cl) and NaH2PO4 aqueous solution in this paper. The partitioning behavior of penicillin G in the ATPS was investigated. Concentrations of NaH2PO4, penicillin G, and [Bmim]Cl were evaluated to determine their effects on the partition coefficient and extraction yield of penicillin G. It was found that both of partition coefficient and extraction yield strongly depended on the concentration of [Bmim]Cl, penicillin and NaH2PO4. A high extraction yield of 93% was achieved with the following parameters: NaH2PO4 center dot 2H(2)O 40% (wt%), penicillin 45000 similar to 50000 u/ml, [Bmim]Cl 20 similar to 21% (wt%). The [Bmim]Cl/NaH2PO4 system was also applied in a real filtration of penicillin G fermentation broth and the extraction yield was averaged at 91.5%. It is worthy noting that the working pH value of ATPS was at the range of 5 similar to 6, no emulsification and protein denaturation could be observed.
WOS标题词	Science & Technology ; Physical Sciences ; Technology
类目[WOS]	Chemistry, Multidisciplinary ; Engineering, Chemical
研究领域[WOS]	Chemistry ; Engineering
关键词[WOS]	ORGANIC-SOLVENTS ; EXTRACTION ; WATER ; BROTH
收录类别	SCI
原文出处	://WOS:000240089800013
语种	英语
WOS记录号	WOS:000240089800013
公开日期	2013-10-24
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/4035]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Chinese Acad Sci, Lab Separat Sci & Engn, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.N China Pharmacuet Grp, Shijiazhuang, Peoples R China
推荐引用方式 GB/T 7714	Liu, Qingfen,Yu, Jiang,Li, Wangliang,et al. Partitioning behavior of penicillin G in aqueous two phase system formed by ionic liquids and phosphate[J]. SEPARATION SCIENCE AND TECHNOLOGY,2006,41(12):2849-2858.
APA	Liu, Qingfen.,Yu, Jiang.,Li, Wangliang.,Hu, Xuesheng.,Xia, Hansong.,...&Yang, Ping.(2006).Partitioning behavior of penicillin G in aqueous two phase system formed by ionic liquids and phosphate.SEPARATION SCIENCE AND TECHNOLOGY,41(12),2849-2858.
MLA	Liu, Qingfen,et al."Partitioning behavior of penicillin G in aqueous two phase system formed by ionic liquids and phosphate".SEPARATION SCIENCE AND TECHNOLOGY 41.12(2006):2849-2858.