Improving recovery of recombinant hepatitis B virus surface antigen by ion-exchange chromatographic supports with low ligand density

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	Improving recovery of recombinant hepatitis B virus surface antigen by ion-exchange chromatographic supports with low ligand density
	Huang, Yongdong ; Bi, Jingxiu ; Zhou, Weibin ; Li, Yan ; Wang, Yangmu ; Ma, Guanghui ; Su, Zhiguo
刊名	PROCESS BIOCHEMISTRY
	2006-11-01
卷号	41 期号:11 页码:2320-2326
关键词	recombinant hepatitis B surface antigen ion-exchange chromatography ligand density multi-point interaction
ISSN号	1359-5113
其他题名	Process Biochem.
中文摘要	Ion-exchange chromatography (IEC) with the lowest step recovery (< 50%) is still a bottleneck of the essentially four-step chromatographic procedure for the purification of recombinant hepatitis B surface antigen (r-HBsAg) from transformed Chinese hamster ovary (CHO) cell line. The low recovery is mainly resulted from the change of the assemble structure of HBsAg particles such as disassociation or aggregation on the surface of the absorbent during binding and eluting procedures. To improve the chromatographic efficiency of IEC, the influence of ligand density from 0.041 to 0.130 mmol diethylaminoethyl (DEAE)/ml absorbent on IEC of r-HBsAg was investigated. The retention time of HBsAg particles depended highly on ligand density. Higher HBsAg recovery and purification factor (PF) were obtained from the absorbents with lower ligand density. Under the optimum chromatographic conditions, the HBsAg recovery was increased from about 44% to 66% and the PF was increased from 9 to 13 by decreasing the ligand density from 0.130 to 0.041 mmol DEAE/ml absorbent. (c) 2006 Elsevier Ltd. All rights reserved.
英文摘要	Ion-exchange chromatography (IEC) with the lowest step recovery (< 50%) is still a bottleneck of the essentially four-step chromatographic procedure for the purification of recombinant hepatitis B surface antigen (r-HBsAg) from transformed Chinese hamster ovary (CHO) cell line. The low recovery is mainly resulted from the change of the assemble structure of HBsAg particles such as disassociation or aggregation on the surface of the absorbent during binding and eluting procedures. To improve the chromatographic efficiency of IEC, the influence of ligand density from 0.041 to 0.130 mmol diethylaminoethyl (DEAE)/ml absorbent on IEC of r-HBsAg was investigated. The retention time of HBsAg particles depended highly on ligand density. Higher HBsAg recovery and purification factor (PF) were obtained from the absorbents with lower ligand density. Under the optimum chromatographic conditions, the HBsAg recovery was increased from about 44% to 66% and the PF was increased from 9 to 13 by decreasing the ligand density from 0.130 to 0.041 mmol DEAE/ml absorbent. (c) 2006 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
研究领域[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
关键词[WOS]	OVARY CELL-LINE ; MAMMALIAN-CELLS ; PURIFICATION ; PARTICLES ; YEAST ; PROTEINS ; RETENTION
收录类别	SCI
原文出处	://WOS:000241474200012
语种	英语
WOS记录号	WOS:000241474200012
公开日期	2013-10-24
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/3995]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Grad Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Beijing Univ Sci & Technol, Dept Environm Sci, Beijing 100083, Peoples R China
推荐引用方式 GB/T 7714	Huang, Yongdong,Bi, Jingxiu,Zhou, Weibin,et al. Improving recovery of recombinant hepatitis B virus surface antigen by ion-exchange chromatographic supports with low ligand density[J]. PROCESS BIOCHEMISTRY,2006,41(11):2320-2326.
APA	Huang, Yongdong.,Bi, Jingxiu.,Zhou, Weibin.,Li, Yan.,Wang, Yangmu.,...&Su, Zhiguo.(2006).Improving recovery of recombinant hepatitis B virus surface antigen by ion-exchange chromatographic supports with low ligand density.PROCESS BIOCHEMISTRY,41(11),2320-2326.
MLA	Huang, Yongdong,et al."Improving recovery of recombinant hepatitis B virus surface antigen by ion-exchange chromatographic supports with low ligand density".PROCESS BIOCHEMISTRY 41.11(2006):2320-2326.