Culture and characteristics of recombinant protein production of an Escherichia coli strain expressing carboxylesterase B1

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	Culture and characteristics of recombinant protein production of an Escherichia coli strain expressing carboxylesterase B1
	Qiao, C. L.; Shen, B. C.; Xing, J. M.; Huang, J.; Zhang, J. L.; Zhao, D. X.; Yang, B.
刊名	INTERNATIONAL BIODETERIORATION & BIODEGRADATION
	2006-09-01
卷号	58 期号:2 页码:77-81
关键词	high-density culture E coli carboxylesterase B1 recombinant gene expression
ISSN号	0964-8305
其他题名	Int. Biodeterior. Biodegrad.
中文摘要	High-density culture was achieved through controlling specific growth rate by limiting glucose concentration to < 0.2gL(-1). Carboxylesterase B I capable of hydrolyzing organophosphate esters was purified from Escherichia coli strain BL21 carrying a cloned esterase B I gene from mosquito. The recombinant strain BL21 carrying pET-ESTB1 was used for the fermentation. Product formation was induced by either a temperature shift from 30 to 42 degrees C or by feeding a mixture of glucose and lactose. Cell growth and production of detoxifying enzyme were affected by oxygen availability. The maximum biomass of E. coli BL21 (pET-ESTB1) increased from 14.9 to 31.5 g dry cell weight l(-1). Using the host strain E. coli BL21 (DE3), detoxifying enzyme was over-expressed at a biomass level of up to 31.5 g dry weight l(-1). The enzyme had a molecular mass of 64 kDa, its optimum temperature was approx. 37 degrees C; at pH 7 the relative activity after 3 h was 85.9% at 28 degrees C, 64.9% at 34 degrees C, and 4.5% at 40 degrees C. The enzyme activity of cells grown at lower temperatures was much higher; at 18 degrees C it almost twice than at 20 or 22 degrees C. degrees 2006 Elsevier Ltd. All rights reserved.
英文摘要	High-density culture was achieved through controlling specific growth rate by limiting glucose concentration to < 0.2gL(-1). Carboxylesterase B I capable of hydrolyzing organophosphate esters was purified from Escherichia coli strain BL21 carrying a cloned esterase B I gene from mosquito. The recombinant strain BL21 carrying pET-ESTB1 was used for the fermentation. Product formation was induced by either a temperature shift from 30 to 42 degrees C or by feeding a mixture of glucose and lactose. Cell growth and production of detoxifying enzyme were affected by oxygen availability. The maximum biomass of E. coli BL21 (pET-ESTB1) increased from 14.9 to 31.5 g dry cell weight l(-1). Using the host strain E. coli BL21 (DE3), detoxifying enzyme was over-expressed at a biomass level of up to 31.5 g dry weight l(-1). The enzyme had a molecular mass of 64 kDa, its optimum temperature was approx. 37 degrees C; at pH 7 the relative activity after 3 h was 85.9% at 28 degrees C, 64.9% at 34 degrees C, and 4.5% at 40 degrees C. The enzyme activity of cells grown at lower temperatures was much higher; at 18 degrees C it almost twice than at 20 or 22 degrees C. degrees 2006 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology ; Environmental Sciences
研究领域[WOS]	Biotechnology & Applied Microbiology ; Environmental Sciences & Ecology
关键词[WOS]	HIGH-CELL-DENSITY ; FED-BATCH CULTURES ; GROWTH ; CULTIVATION ; POLLUTANTS ; GLUCOSE
收录类别	ISTP ; SCI
原文出处	://WOS:000242779600005
语种	英语
WOS记录号	WOS:000242779600005
公开日期	2013-10-24
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/3944]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Acad Sinica, Inst Zool, State Key Lab Integrated Management Pest Insects, Beijing 100080, Peoples R China 2.Acad Sinica, Inst Proc Engn, Beijing 100080, Peoples R China 3.Acad Sinica, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Qiao, C. L.,Shen, B. C.,Xing, J. M.,et al. Culture and characteristics of recombinant protein production of an Escherichia coli strain expressing carboxylesterase B1[J]. INTERNATIONAL BIODETERIORATION & BIODEGRADATION,2006,58(2):77-81.
APA	Qiao, C. L..,Shen, B. C..,Xing, J. M..,Huang, J..,Zhang, J. L..,...&Yang, B..(2006).Culture and characteristics of recombinant protein production of an Escherichia coli strain expressing carboxylesterase B1.INTERNATIONAL BIODETERIORATION & BIODEGRADATION,58(2),77-81.
MLA	Qiao, C. L.,et al."Culture and characteristics of recombinant protein production of an Escherichia coli strain expressing carboxylesterase B1".INTERNATIONAL BIODETERIORATION & BIODEGRADATION 58.2(2006):77-81.