Carrier-Free Immobilization of Rutin Degrading Enzyme Extracted From Fusarium spp.

doi:10.3389/fbioe.2020.00470

CORC > 兰州理工大学 > 兰州理工大学

	Carrier-Free Immobilization of Rutin Degrading Enzyme Extracted From Fusarium spp.
	Zang, Yuan-Yuan 2; Yang, Sha 2; Xu, Yong-Qiang 1; Chen, Zhi-Gang 2; Wu, Tao 3
刊名	Frontiers in Bioengineering and Biotechnology
	2020-06-26
卷号	8
关键词	Alcohols Degradation Enzyme activity Fungi Hydrolysis Deep eutectic solvents Enzyme characteristics Hydrophobic environment Immobilization method Industrial production Optimal conditions Orthogonal experiment Tartary buckwheat
ISSN号	2296-4185
DOI	10.3389/fbioe.2020.00470
英文摘要	In this study, a strain with rutin degrading enzyme (RDE) activity was screened from moldy tartary buckwheat and subsequently identified as Fusarium spp. The structure and enzyme characteristics of CLEA-RDE formed by immobilization via cross-linking were then investigated. Further, the optimal catalysis conditions of CLEA-RDE in natural deep eutectic solvents (NADESs) serving as hydrolysis solvents were also investigated. The results of SEM and spectrum indicated that CLEA-RDE became more stable than free-RDE due to the cross-linking. Interestingly, CLEA-RDE showed a wider range of pH adaptation and higher tolerance to low temperatures (20 – 30°C) and hydrophobic environments. The results of orthogonal experiments revealed that the optimal condition for rutin hydrolysis was under pH 5.0 and 40oC with the degradation rate of 10.65 mg min−1 L−1. The preparation of CLEA-RDE without a carrier-based immobilization method reduces the loss of enzyme activity, improves the stability of the enzyme and can be applied to the investigation of immobilization of various enzymes, thus providing a referred idea for the improvement of catalysts in industrial production. © Copyright © 2020 Zang, Yang, Xu, Chen and Wu.
WOS研究方向	Biotechnology & Applied Microbiology ; Science & Technology - Other Topics
语种	英语
出版者	Frontiers Media S.A.
WOS记录号	WOS:000551729200001
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/115709]
专题	兰州理工大学
作者单位	1.College of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, China; 2.Glycomics and Glycan Bioengineering Research Center, College of Food Science & Technology, Nanjing Agricultural University, Nanjing, China; 3.Department of Food Science, University of Tennessee, Knoxville, Knoxville; TN, United States
推荐引用方式 GB/T 7714	Zang, Yuan-Yuan,Yang, Sha,Xu, Yong-Qiang,et al. Carrier-Free Immobilization of Rutin Degrading Enzyme Extracted From Fusarium spp.[J]. Frontiers in Bioengineering and Biotechnology,2020,8.
APA	Zang, Yuan-Yuan,Yang, Sha,Xu, Yong-Qiang,Chen, Zhi-Gang,&Wu, Tao.(2020).Carrier-Free Immobilization of Rutin Degrading Enzyme Extracted From Fusarium spp..Frontiers in Bioengineering and Biotechnology,8.
MLA	Zang, Yuan-Yuan,et al."Carrier-Free Immobilization of Rutin Degrading Enzyme Extracted From Fusarium spp.".Frontiers in Bioengineering and Biotechnology 8(2020).