Aqueous two-phase extraction of polysaccharide from Potentilla anserine L.

doi:10.19026/ajfst.9.1665

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	Aqueous two-phase extraction of polysaccharide from Potentilla anserine L.
	Ma, Hui; Wei, Jun-Qing; Su, Dan; Guo, Tao; Feng, Zai-Ping; Zhang, Ju-Bao; Tan, Su-Bei; Wei, Li-Yang
刊名	Advance Journal of Food Science and Technology
	2015
卷号	9 期号:10 页码:807-811
关键词	Ethanol Polysaccharides Solvent extraction Aqueous two phase system Aqueous two-phase extraction Conventional extraction Conventional methods Extraction yield Optimal process Partition coefficient Potentilla anserina
ISSN号	20424868
DOI	10.19026/ajfst.9.1665
英文摘要	Aqueous two-phase system is a useful method for purifying bio-logical materials in biochemistry and biotechnology. In this study the potential use of a K2HPO4/ethanol aqueous two-phase system to extract polysaccharides from Potentilla anserine L. The effects of salt, ethanol, NaCl concentrate and pH on the partitioning of polysaccharides were studied. The ATPS with 27% ethanol, 21% K2HPO4, 0.4 mol/L NaCl and pH 7 were selected as the optimal process system according to the partition coefficient and recovery. Furthermore, a comparison between the ATPS and the conventional extraction was conducted. For ATPS, the extraction yield and purity were 12.47 and 16.18%; while the extraction yield and purity were 10.3 and 6.14% by conventional extraction, respectively. ATPS showed a higher the extraction yield and the purity was around 2-fold with conventional method. So the ATPS can be employed to become an efficient and prospective process for the extraction. © 2015 Maxwell Scientific Publication Corp.
语种	英语
出版者	Maxwell Science Publications
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/112328]
专题	兰州理工大学
作者单位	Department of Gansu Education, The Key Lab of Screening, Evaluation and Advanced Processing of TCM and Tibetan Medicine, School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, China
推荐引用方式 GB/T 7714	Ma, Hui,Wei, Jun-Qing,Su, Dan,et al. Aqueous two-phase extraction of polysaccharide from Potentilla anserine L.[J]. Advance Journal of Food Science and Technology,2015,9(10):807-811.
APA	Ma, Hui.,Wei, Jun-Qing.,Su, Dan.,Guo, Tao.,Feng, Zai-Ping.,...&Wei, Li-Yang.(2015).Aqueous two-phase extraction of polysaccharide from Potentilla anserine L..Advance Journal of Food Science and Technology,9(10),807-811.
MLA	Ma, Hui,et al."Aqueous two-phase extraction of polysaccharide from Potentilla anserine L.".Advance Journal of Food Science and Technology 9.10(2015):807-811.