Efficient production of succinic acid from corn stalk hydrolysates by a recombinant Escherichia coli with ptsG mutation

CORC > 过程工程研究所 > 中国科学院过程工程研究所 > 生化工程国家重点实验室

	Efficient production of succinic acid from corn stalk hydrolysates by a recombinant Escherichia coli with ptsG mutation
	Wang, Dan 1,2; Li, Qiang 1,2; Yang, Maohua 1,2; Zhang, Yunjian 3; Su, Zhiguo 1; Xing, Jianmin 1
刊名	PROCESS BIOCHEMISTRY
	2011
卷号	46 期号:1 页码:365-371
关键词	Glucose phosphotransferase Escherichia coli Succinic acid Phosphoenolpyruvate carboxylase Corn stalk hydrolysates
ISSN号	1359-5113
通讯作者	Xing, JM
英文摘要	Succinic acid is considered to be one of the key platform chemicals used in a variety of industrial applications. The exploitation of biomass to produce succinic acid requires a microbial type that can ferment the mixture of reducing sugars derived from lignocellulose. The recombinant Escherichia coli strains with homologous or cyanobacterial ppc overexpression and IdhA, pflB, ptsG mutations were constructed, and the mixed sugar fermentations were carried out with the prominent strain SD121. Then, a modeled corn stalk hydrolysates containing 30 g l(-1) glucose, 10 g l(-1) xylose and 2.5 g l(-1) arabinose was applied for succinic acid fermentation with SD121. A yield of 0.77 g succinic acid g(-1) total sugar was achieved. Fermentation of corn stalk hydrolysates with SD121 produced a final succinic acid concentration of 36.55 g l(-1) with a higher yield of 0.83 g g(-1) total sugar in anaerobic bottles. In two-stage fermentation process in bioreactor, initial aerobic growth facilitated the subsequent anaerobic succinic acid production with a final concentration of 57.81 g l(-1), and a yield of 0.87 g g(-1), total sugar. This was the first report of succinic acid production from corn stalk hydrolysates by metabolically engineered Escherichia coli. The higher succinic acid yield from corn stalk hydrolysates compared to modeled sugar mixtures, showed a great potential usage of renewable biomass as a feedstock for an economical succinic acid production using E. coli. (C) 2010 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
研究领域[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
关键词[WOS]	CATABOLITE REPRESSION MUTANTS ; PHOSPHOENOLPYRUVATE CARBOXYLASE ; ACTINOBACILLUS-SUCCINOGENES ; LACTATE-DEHYDROGENASE ; PYRUVATE-CARBOXYLASE ; SUGAR MIXTURES ; MALIC ENZYME ; FERMENTATION ; EXPRESSION ; OVEREXPRESSION
收录类别	SCI
语种	英语
WOS记录号	WOS:000286538300051
公开日期	2013-11-08
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/5253]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.China Univ Min & Technol Beijing, Dept Bioengn, Sch Chem & Environm Engn, Beijing 100083, Peoples R China
推荐引用方式 GB/T 7714	Wang, Dan,Li, Qiang,Yang, Maohua,et al. Efficient production of succinic acid from corn stalk hydrolysates by a recombinant Escherichia coli with ptsG mutation[J]. PROCESS BIOCHEMISTRY,2011,46(1):365-371.
APA	Wang, Dan,Li, Qiang,Yang, Maohua,Zhang, Yunjian,Su, Zhiguo,&Xing, Jianmin.(2011).Efficient production of succinic acid from corn stalk hydrolysates by a recombinant Escherichia coli with ptsG mutation.PROCESS BIOCHEMISTRY,46(1),365-371.
MLA	Wang, Dan,et al."Efficient production of succinic acid from corn stalk hydrolysates by a recombinant Escherichia coli with ptsG mutation".PROCESS BIOCHEMISTRY 46.1(2011):365-371.