One-step separation and purification of hydrolysable tannins from Geranium wilfordii Maxim by adsorption chromatography on cross-linked 12% agarose gel

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	One-step separation and purification of hydrolysable tannins from Geranium wilfordii Maxim by adsorption chromatography on cross-linked 12% agarose gel
	Liu, Dan 2; Ma, Yan 3; Wang, Ye 4; Su, Zhiguo 1; Gu, Ming 1; Janson, Jan-Christer 5
刊名	JOURNAL OF SEPARATION SCIENCE
	2011-05-01
卷号	34 期号:9 页码:995-998
关键词	Adsorption chromatography Cross-linked agarose gel Hydrolysable tannin
ISSN号	1615-9306
通讯作者	Gu, M
英文摘要	The hydrolysable tannins corilagin and geraniin, the major active components of the traditional Chinese medicine Geranium wilfordii Maxim, have been separated and purified from crude extracts in one step by adsorption chromatography on cross-linked 12% agarose gel (Superose 12 10/300 GL). The separation was achieved by gradient elution using mobile phase A composed of 5% ethanol and 5% acetic acid and mobile phase B composed of 30% ethanol and 30% acetic acid. The gradients were composed as follows: 0-240 mL, 0-25% B; 240-480 mL, 25-40% B; after 480 mL, 100% B. The purities of the collected corilagin and geraniin were 92.4 and 87.2%, and the corresponding yields were 88.0 and 76.8%, respectively.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Analytical
研究领域[WOS]	Chemistry
关键词[WOS]	CENTRIFUGAL PARTITION CHROMATOGRAPHY ; COUNTER-CURRENT CHROMATOGRAPHY ; CORILAGIN ; MEDIA ; ACID ; L. ; POLYPHENOLS ; ANTIOXIDANT ; CHEMISTRY ; GALLATE
收录类别	SCI
语种	英语
WOS记录号	WOS:000290634600004
公开日期	2013-11-05
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/4883]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Dalian Univ Technol, Sch Environm & Biol Sci & Technol, Dalian, Peoples R China 3.Jiangsu Membrane Separat Co Ltd, Dalian, Peoples R China 4.Nanjing Agr Univ, Nanjing, Peoples R China 5.Uppsala Univ, Uppsala, Sweden
推荐引用方式 GB/T 7714	Liu, Dan,Ma, Yan,Wang, Ye,et al. One-step separation and purification of hydrolysable tannins from Geranium wilfordii Maxim by adsorption chromatography on cross-linked 12% agarose gel[J]. JOURNAL OF SEPARATION SCIENCE,2011,34(9):995-998.
APA	Liu, Dan,Ma, Yan,Wang, Ye,Su, Zhiguo,Gu, Ming,&Janson, Jan-Christer.(2011).One-step separation and purification of hydrolysable tannins from Geranium wilfordii Maxim by adsorption chromatography on cross-linked 12% agarose gel.JOURNAL OF SEPARATION SCIENCE,34(9),995-998.
MLA	Liu, Dan,et al."One-step separation and purification of hydrolysable tannins from Geranium wilfordii Maxim by adsorption chromatography on cross-linked 12% agarose gel".JOURNAL OF SEPARATION SCIENCE 34.9(2011):995-998.