A novel role of 'pseudo' gamma-butyrolactone receptors in controlling gamma-butyrolactone biosynthesis in Streptomyces

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	A novel role of 'pseudo' gamma-butyrolactone receptors in controlling gamma-butyrolactone biosynthesis in Streptomyces
	Wang, Juan 1; Wang, Weishan 1; Wang, Linqi 1; Zhang, Guifeng 2; Fan, Keqiang 1; Tan, Huarong 1; Yang, Keqian 1
刊名	MOLECULAR MICROBIOLOGY
	2011-10-01
卷号	82 期号:1 页码:236-250
关键词	FAMILY TRANSCRIPTIONAL REGULATOR ANTIBIOTIC PRODUCTION SECONDARY METABOLISM A-FACTOR COELICOLOR A3(2) MORPHOLOGICAL-DIFFERENTIATION GENE-CLUSTER VENEZUELAE ISP5230 MICROBIAL HORMONE AUTOREGULATOR
ISSN号	0950-382X
通讯作者	Yang, KQ
英文摘要	In streptomycetes, a quorum-sensing mechanism mediated by gamma-butyrolactones (GBLs) and their cognate receptors was known to trigger secondary metabolism and morphological differentiation. However, many aspects on the control of GBL signal production are not understood. In this work, we report that ScbR2, the pseudo GBL receptor in Streptomyces coelicolor, negatively controls the biosynthesis of gamma-butyrolactone (SCB1) by directly repressing the transcription of scbA, which encodes the key enzyme for SCB1 biosynthesis. Similarly, the pseudo GBL receptor JadR2 in Streptomyces venezuelae was shown to repress the expression of jadW1, which also encodes the putative GBL synthase. These regulatory relationships were verified in Escherichia coli using lux-based reporter constructs. Additionally, the temporal expression profiles of scbA, scbR2 and scbR (receptor gene for SCB1) were examined in Streptomyces coelicolor, which showed the sequential expression of ScbR/R2 regulators in the control of SCB1 production. Overall, our results clearly demonstrated that pseudo GBL receptors play a novel role in controlling GBL biosynthesis in streptomycetes. As ScbR/R2 homologues and their binding sites upstream of GBL synthase genes are commonly found in Streptomyces species, and ScbR2 homologues cross-recognize each other's target promoters, the ScbA/R/R2 quorum-sensing regulatory system appears to represent an evolutionarily conserved signal control mechanism.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Microbiology
研究领域[WOS]	Biochemistry & Molecular Biology ; Microbiology
关键词[WOS]	FAMILY TRANSCRIPTIONAL REGULATOR ; ANTIBIOTIC PRODUCTION ; SECONDARY METABOLISM ; A-FACTOR ; COELICOLOR A3(2) ; MORPHOLOGICAL-DIFFERENTIATION ; GENE-CLUSTER ; VENEZUELAE ISP5230 ; MICROBIAL HORMONE ; AUTOREGULATOR
收录类别	SCI
语种	英语
WOS记录号	WOS:000295226700018
公开日期	2013-10-31
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/4624]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wang, Juan,Wang, Weishan,Wang, Linqi,et al. A novel role of 'pseudo' gamma-butyrolactone receptors in controlling gamma-butyrolactone biosynthesis in Streptomyces[J]. MOLECULAR MICROBIOLOGY,2011,82(1):236-250.
APA	Wang, Juan.,Wang, Weishan.,Wang, Linqi.,Zhang, Guifeng.,Fan, Keqiang.,...&Yang, Keqian.(2011).A novel role of 'pseudo' gamma-butyrolactone receptors in controlling gamma-butyrolactone biosynthesis in Streptomyces.MOLECULAR MICROBIOLOGY,82(1),236-250.
MLA	Wang, Juan,et al."A novel role of 'pseudo' gamma-butyrolactone receptors in controlling gamma-butyrolactone biosynthesis in Streptomyces".MOLECULAR MICROBIOLOGY 82.1(2011):236-250.