LiCl-induced improvement of multilayer nanofibrous lipase for biodiesel synthesis

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	LiCl-induced improvement of multilayer nanofibrous lipase for biodiesel synthesis
	Liu, Chun-Xia 1,2; Zhang, Song-Ping 1; Su, Zhi-Guo 1; Wang, Ping 3,4
刊名	BIORESOURCE TECHNOLOGY
	2012
卷号	103 期号:1 页码:266-272
关键词	Biodiesel Lipase AK Polyurethane nanofibers LiCl Stability
ISSN号	0960-8524
通讯作者	Zhang, SP
英文摘要	A unique method that applied a multilayer-immobilization strategy was developed to prepare nanofibrous enzymes for biosynthesis. LiCl co-electrospun with polyurethane nanofibers enabled strong physical adsorption of bovine serum albumin (BSA), forming the first layer of protein on the nanofibers; lipase AK was subsequently crosslinked to BSA as an outer layer of enzyme. The content of LiCl in nanofibers was found to be a sensitive factor affecting the activity and stability of the immobilized lipase. For bio-diesel synthesis from soybean oil and methanol in isooctane, the reaction rate catalyzed by nanofibrious lipase carrying 5 wt% LiCl was 6.6-fold higher than fibers without LiCl, with a conversion of 91% was achieved within 2 h. LiCl also induced much improved enzyme stability. The nanofibrous lipase with 5% LiCl could be repeatedly used for 42 cycles without apparent activity loss, while the immobilized lipase without LiCl lost over 90% activity within 13 reuse cycles. (C) 2011 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]	Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
研究领域[WOS]	Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
关键词[WOS]	CANDIDA-RUGOSA LIPASE ; ORGANIC-SOLVENTS ; ESTERIFICATION ACTIVITY ; CEPACIA LIPASE ; ENZYME ; STABILITY ; POLYMER ; CONFORMATION ; ADSORPTION ; INTERFACES
收录类别	SCI
语种	英语
WOS记录号	WOS:000298273600036
公开日期	2013-10-29
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/4342]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Univ Minnesota, Inst Biotechnol, St Paul, MN 55108 USA 4.Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA
推荐引用方式 GB/T 7714	Liu, Chun-Xia,Zhang, Song-Ping,Su, Zhi-Guo,et al. LiCl-induced improvement of multilayer nanofibrous lipase for biodiesel synthesis[J]. BIORESOURCE TECHNOLOGY,2012,103(1):266-272.
APA	Liu, Chun-Xia,Zhang, Song-Ping,Su, Zhi-Guo,&Wang, Ping.(2012).LiCl-induced improvement of multilayer nanofibrous lipase for biodiesel synthesis.BIORESOURCE TECHNOLOGY,103(1),266-272.
MLA	Liu, Chun-Xia,et al."LiCl-induced improvement of multilayer nanofibrous lipase for biodiesel synthesis".BIORESOURCE TECHNOLOGY 103.1(2012):266-272.