Computational Fluid Dynamics Modeling of an Inverted Frusto-conical Shaking Bioreactor for Mammalian Cell Suspension Culture

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	Computational Fluid Dynamics Modeling of an Inverted Frusto-conical Shaking Bioreactor for Mammalian Cell Suspension Culture
	Hang, Haifeng 1; Guo, Yuanxin 1; Liu, Jian 1; Bai, Li 1; Xia, Jianye 1; Guo, Meijin 1; Hui, Matthew 2
刊名	BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
	2011-06-01
卷号	16 期号:3 页码:567-575
关键词	inverted frusto-conical shaking bioreactor mammalian cell suspension culture mixing time volumetric oxygen transfer coefficient computational fluid dynamics simulation
ISSN号	1226-8372
通讯作者	Guo, MJ
英文摘要	We previously developed an inverted frusto-conical shaking bioreactor (IFSB) which had high mammalian cell culture performance when compared with a mechanically stirred tank reactor (STR) or a flat-bottom shaking bioreactor (FBSB). Here, we determined the mixing time (t) and volumetric oxygen transfer coefficient (k(L)a) of this IFSB at various speeds, and simulated the fluid hydrodynamics, including the shear stress and specific surface area, by computational fluid dynamics. The shortest mixing time was observed in a STR. The maximum k(L)a value of 12/h was achieved in the IFSB at an aeration rate of 4 L/h, demonstrating that our IFSB has enhanced oxygen transfer capabilities needed to meet the demands of mammalian cells. Simulation studies revealed a 3% greater specific surface area and a 21% lower shear strain in the IFSB compared to an FBSB under the same conditions. Additionally, the conical angle of the vessel, which significantly affected cell growth and recombinant protein production, was tested here. We conclude that, compared to the STR and FBSB, the IFSB has an increased liquid surface area for oxygen uptake and exhaust CO2 stripping, an enhanced k(L)a for cell robust growth to a high cell density, and a lower shear stress to alleviate cell damage.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
收录类别	SCI
语种	英语
WOS记录号	WOS:000292204700025
公开日期	2013-10-29
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/4341]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Hang, Haifeng,Guo, Yuanxin,Liu, Jian,et al. Computational Fluid Dynamics Modeling of an Inverted Frusto-conical Shaking Bioreactor for Mammalian Cell Suspension Culture[J]. BIOTECHNOLOGY AND BIOPROCESS ENGINEERING,2011,16(3):567-575.
APA	Hang, Haifeng.,Guo, Yuanxin.,Liu, Jian.,Bai, Li.,Xia, Jianye.,...&Hui, Matthew.(2011).Computational Fluid Dynamics Modeling of an Inverted Frusto-conical Shaking Bioreactor for Mammalian Cell Suspension Culture.BIOTECHNOLOGY AND BIOPROCESS ENGINEERING,16(3),567-575.
MLA	Hang, Haifeng,et al."Computational Fluid Dynamics Modeling of an Inverted Frusto-conical Shaking Bioreactor for Mammalian Cell Suspension Culture".BIOTECHNOLOGY AND BIOPROCESS ENGINEERING 16.3(2011):567-575.