Deliberate manipulation of the surface hydrophobicity of an adsorbent for an efficient purification of a giant molecule with multiple subunits

CORC > 过程工程研究所 > 中国科学院过程工程研究所 > 生化工程国家重点实验室

	Deliberate manipulation of the surface hydrophobicity of an adsorbent for an efficient purification of a giant molecule with multiple subunits
	Zhao, Lan 1,2; Liu, Yongdong 1; Huang, Yongdong 1; Li, Xiunan 1; Wang, Yinjue 1; Li, Yan 1; Ma, Guanghui 1; Su, Zhiguo 1
刊名	JOURNAL OF SEPARATION SCIENCE
	2011-11-01
卷号	34 期号:22 页码:3186-3193
关键词	Hydrophobic interaction chromatography Ligand density Purification rHBsAg Spacer arm
ISSN号	1615-9306
通讯作者	Su, ZG
英文摘要	Hydrophobic interaction chromatography (HIC) is often an inevitable step for a satisfying purification in giant vaccine molecules production. But great mass and activity loss associated with poor purity often occur simultaneously. In this paper, high purity and high bioactivity recovery for the HIC process of hepatitis B surface antigen (rHBsAg) purification were achieved through manipulation of surface hydrophobicity of the adsorbent. Spacer arm length and ligand density were regulated, respectively, through which the interaction between the vaccine and the adsorbent was manipulated deliberately. It was found even in a narrow scope, varying spacer arm length and ligand density resulted in purification factor changing from 1 to 96.5, and rHBsAg recovery from 3 to 91%. The optimal purification performance was achieved when the spacer arm was C8 and the ligand density was 9.2 mu mol/g suction-dried wet gel with an average distance of ligands of 3.6 nm. This deliberate regulation strategy represents a new approach of improving purification of giant multi-subunit proteins.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Analytical
研究领域[WOS]	Chemistry
关键词[WOS]	LASER-SCANNING MICROSCOPY ; CHINESE-HAMSTER OVARY ; HEPATITIS-B VACCINES ; HANSENULA-POLYMORPHA ; PICHIA-PASTORIS ; LIGAND DENSITY ; SPACER ARM ; ANTIGEN ; RECOMBINANT ; CHROMATOGRAPHY
收录类别	SCI
语种	英语
WOS记录号	WOS:000297949400010
公开日期	2013-10-28
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/4339]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Lan,Liu, Yongdong,Huang, Yongdong,et al. Deliberate manipulation of the surface hydrophobicity of an adsorbent for an efficient purification of a giant molecule with multiple subunits[J]. JOURNAL OF SEPARATION SCIENCE,2011,34(22):3186-3193.
APA	Zhao, Lan.,Liu, Yongdong.,Huang, Yongdong.,Li, Xiunan.,Wang, Yinjue.,...&Su, Zhiguo.(2011).Deliberate manipulation of the surface hydrophobicity of an adsorbent for an efficient purification of a giant molecule with multiple subunits.JOURNAL OF SEPARATION SCIENCE,34(22),3186-3193.
MLA	Zhao, Lan,et al."Deliberate manipulation of the surface hydrophobicity of an adsorbent for an efficient purification of a giant molecule with multiple subunits".JOURNAL OF SEPARATION SCIENCE 34.22(2011):3186-3193.