Thermal-sensitive hydrogel as adjuvant-free vaccine delivery system for H5N1 intranasal immunization

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	Thermal-sensitive hydrogel as adjuvant-free vaccine delivery system for H5N1 intranasal immunization
	Wu, Youbin 1,2; Wei, Wei 1; Zhou, Meng 1; Wang, Yueqi 1,2; Wu, Jie 1; Ma, Guanghui 1; Su, Zhiguo 1
刊名	BIOMATERIALS
	2012-03-01
卷号	33 期号:7 页码:2351-2360
关键词	Thermal-sensitive hydrogel Intranasal immunization Vaccine delivery system Adjuvant-free H5N1 split antigen
ISSN号	0142-9612
通讯作者	Wu, J
英文摘要	For H5N1 influenza immunization, we developed a thermal-sensitive hydrogel as intranasal vaccine delivery system, which was formulated with N-[(2-hydroxy-3-trimethylammonium) propyl] chitosan chloride (HTCC) and alpha, beta-glycerophosphate (alpha, beta-GP). The flowing solution of HTCC/CP under room temperature could gelate rapidly at body temperature, which significantly prolonged the H5N1 split antigen residence time in nasal cavity. This system also enhanced the transepithelial transport via the paracellular routes due to the disorganization of ZO-1 protein in nasal epithelial tissue. In comparison to naked H5N1 split antigen and MF59 adjuvanted antigen, as designed hydrogel/H5N1 vaccine induced greater antigen-specific systemic immune responses and mucosal IgA immunity without adjuvants. Furthermore, a boosted cellular and humoral response was also obtained by examination of IFN-gamma and IL-4 cytokines, respectively. In addition, hydrogel based formulation promoted the antigen-specific CD8(+) T cell immune memory as determined by the proportion of central and effector memory CD8(+) T cells in nasal associated lymphoid tissue (NALT). These results demonstrate that the HTCC hydrogel has potential as an adjuvant-free platform for H5N1 split antigen intranasal vaccination. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Technology
类目[WOS]	Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]	Engineering ; Materials Science
关键词[WOS]	NASAL DRUG-DELIVERY ; INFLUENZA VACCINE ; MUCOSAL IMMUNITY ; CHITOSAN ; INDUCTION ; MICE ; IMMUNOGENICITY ; GENERATION ; EPITHELIUM ; CHALLENGE
收录类别	SCI
语种	英语
WOS记录号	WOS:000300473900043
公开日期	2013-10-26
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/4289]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wu, Youbin,Wei, Wei,Zhou, Meng,et al. Thermal-sensitive hydrogel as adjuvant-free vaccine delivery system for H5N1 intranasal immunization[J]. BIOMATERIALS,2012,33(7):2351-2360.
APA	Wu, Youbin.,Wei, Wei.,Zhou, Meng.,Wang, Yueqi.,Wu, Jie.,...&Su, Zhiguo.(2012).Thermal-sensitive hydrogel as adjuvant-free vaccine delivery system for H5N1 intranasal immunization.BIOMATERIALS,33(7),2351-2360.
MLA	Wu, Youbin,et al."Thermal-sensitive hydrogel as adjuvant-free vaccine delivery system for H5N1 intranasal immunization".BIOMATERIALS 33.7(2012):2351-2360.