Novel in situ product removal technique for simultaneous production of propionic acid and vitamin B12 by expanded bed adsorption bioreactor

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	Novel in situ product removal technique for simultaneous production of propionic acid and vitamin B12 by expanded bed adsorption bioreactor
	Wang, Peng ; Wang, Yunshan 1; Liu, Yongdong ; Shi, Hong ; Su, Zhiguo
刊名	BIORESOURCE TECHNOLOGY
	2012
卷号	104 期号:0 页码:652-659
关键词	Propionibacterium freudenreichii Propionic acid Vitamin B12 In situ product removal Expanded bed
ISSN号	0960-8524
通讯作者	Wang, YS
英文摘要	A new type of in situ product removal (ISPR) technique of expanded bed adsorption (EBA) bioreactor was studied to simultaneously produce extracellular propionic acid and intracellular vitamin B12 by Propionibacterium freudenreichii CICC 10019. Resin screening experiments showed that the ZGA330 resin have the best biocompatibility and highest adsorption for propionic acid. Through the EBA bioreactor, propionic acid could be recovered efficiently by semi-continuous recirculation of the unfiltered broth, which eliminated the feedback inhibition of propionic acid. Fed-batch fermentation was carried out using the EBA system, resulting in a propionic acid concentration of 52.5 g L-1 and vitamin B12 concentration of 43.04 mg L-1 at 160h, which correspond to product yields of 0.66 g g(-1) and 0.54 mg g(-1), respectively. The present study suggests that the EBA bioreactor can be utilized for the simple and economical production of propionic acid and vitamin B12 in a single fermentation process. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]	Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
研究领域[WOS]	Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
关键词[WOS]	ION-EXCHANGE RESIN ; PROPIONIBACTERIUM-ACIDIPROPIONICI ; BATCH FERMENTATION ; LACTIC-ACID ; RECOVERY
收录类别	SCI
语种	英语
WOS记录号	WOS:000301155800088
公开日期	2013-10-23
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/3908]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Peng,Wang, Yunshan,Liu, Yongdong,et al. Novel in situ product removal technique for simultaneous production of propionic acid and vitamin B12 by expanded bed adsorption bioreactor[J]. BIORESOURCE TECHNOLOGY,2012,104(0):652-659.
APA	Wang, Peng,Wang, Yunshan,Liu, Yongdong,Shi, Hong,&Su, Zhiguo.(2012).Novel in situ product removal technique for simultaneous production of propionic acid and vitamin B12 by expanded bed adsorption bioreactor.BIORESOURCE TECHNOLOGY,104(0),652-659.
MLA	Wang, Peng,et al."Novel in situ product removal technique for simultaneous production of propionic acid and vitamin B12 by expanded bed adsorption bioreactor".BIORESOURCE TECHNOLOGY 104.0(2012):652-659.