Improvement of Adenosylcobalamin Production by Metabolic Control Strategy in Propionibacterium freudenreichii

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	Improvement of Adenosylcobalamin Production by Metabolic Control Strategy in Propionibacterium freudenreichii
	Wang, Peng 1,2; Wang, Yunshan 1,2; Su, Zhiguo 1,2
刊名	APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
	2012-05-01
卷号	167 期号:1 页码:62-72
关键词	Biosynthesis Adenosylcobinamide Metabolism control 5 Propionibacterium freudenreichii 6-Dimethylbenzimidazole
ISSN号	0273-2289
通讯作者	Wang, YS
英文摘要	An efficient metabolic control approach was developed to improve the industrial anaerobic fermentation of adenosylcobalamin (ado-cbl) by . The effects of 5,6-dimethylbenzimidazole (DMB) on cell growth and ado-cbl biosynthesis were investigated. Subsequently, the results obtained from the batch culture showed that an important intermediate of ado-cbl separated from the cell extract was identified as adenosylcobinamide (ado-cbi) by high-performance liquid chromatography coupled to an ultraviolet diode array detector and ESI mass spectrometry analysis. Ado-cbi can be converted to ado-cbl when linked to DMB, which is an essential compound for ado-cbi bioconversion. This key ado-cbi is useful not only in determining ado-cbl concentration in the fermentation process but also in serving as an effective compound to guide DMB incorporation for the harvest of the maximum ado-cbl concentration. Accordingly, with scaling up to 100 L fermentation, the experimental results showed that the discrepancy was less than 1 % using the developed prediction technique. Overall, the findings show that the method is efficient in evaluating the fermentation of ado-cbl by propionibacteria.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
研究领域[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
关键词[WOS]	ACID FERMENTATION ; ESCHERICHIA-COLI ; VITAMIN-B-12 ; ENCODES ; ENZYME ; GENOME ; GENE
收录类别	SCI
语种	英语
WOS记录号	WOS:000304204200006
公开日期	2013-10-17
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/3790]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Peng,Wang, Yunshan,Su, Zhiguo. Improvement of Adenosylcobalamin Production by Metabolic Control Strategy in Propionibacterium freudenreichii[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2012,167(1):62-72.
APA	Wang, Peng,Wang, Yunshan,&Su, Zhiguo.(2012).Improvement of Adenosylcobalamin Production by Metabolic Control Strategy in Propionibacterium freudenreichii.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,167(1),62-72.
MLA	Wang, Peng,et al."Improvement of Adenosylcobalamin Production by Metabolic Control Strategy in Propionibacterium freudenreichii".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 167.1(2012):62-72.