Degradation of pentachlorophenol by a novel peroxidase-catalyzed process in the presence of reduced nicotinamide adenine dinucleotide

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	Degradation of pentachlorophenol by a novel peroxidase-catalyzed process in the presence of reduced nicotinamide adenine dinucleotide
	Li, Haitao 1,2; Li, Yuping 1; Cao, Hongbin 1; Li, Xingang 2; Zhang, Yi 1
刊名	CHEMOSPHERE
	2011-03-01
卷号	83 期号:2 页码:124-130
关键词	Horseradish peroxidase Pentachlorophenol Hydroxyl radicals Superoxide anion radical Electron paramagnetic resonance NADH
ISSN号	0045-6535
通讯作者	Li, YP
英文摘要	A novel horseradish peroxidase (HRP)-catalyzed H(2)O(2) process in the presence of reduced nicotinamide adenine dinucleotide (NADH) was applied to remove aqueous pentachlorophenol (PCP). Parameters (PH, H(2)O(2) concentration, HRP activity and NADH dosage) on PCP removal were investigated. It was found that initial 0.05 mM PCP was removed by 98% in HRP-NADH-H(2)O(2) system at pH 5.0 and 30 degrees C for 1 h. Addition of O(2) in HRP-NADH-H(2)O(2) system enhanced the removal rate of PCP due to promoting hydroxyl radicals (center dot OH) and superoxide anion radical) generation, which were confirmed by electron paramagnetic resonance (EPR)-spin trapping method. PCP removal efficiency decreased when center dot O ((2)) over bar and H(2)O(2) were scavenged by superoxide dismutase and catalase in HRP-NADH-O(2) system, indicating that center dot OH/center dot O ((2)) over bar played a great role in the degradation of PCP. Gas chromatography-mass spectrometry (GC-MS) analysis revealed that octachlorinated dibenzodioxin (OCDD) in residual solution was reduced after treated by the HRP-NADH-O(2) process, resulting in lower toxicity of treated solution than conventional enzymatic process. Two enzymatic-catalysis pathways were proposed for PCP removal in HRP-NADH-H(2)O(2)/O(2) system: (i) center dot OH/center dot O ((2)) over bar free radical oxidation (ii) conventional phenoxy polymerization. (C) 2011 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Environmental Sciences
研究领域[WOS]	Environmental Sciences & Ecology
关键词[WOS]	GENERATED HYDROXYL RADICALS ; DIBENZO-P-DIOXINS ; HORSERADISH-PEROXIDASE ; HYDROGEN-PEROXIDE ; AQUEOUS PENTACHLOROPHENOL ; OXIDATION ; REMOVAL ; ENZYME ; PCP ; PHENOLS
收录类别	SCI
语种	英语
WOS记录号	WOS:000288926400005
公开日期	2013-11-06
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/5064]
专题	过程工程研究所_湿法冶金清洁生产技术国家工程实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China 2.Tianjin Univ, Sch Chem Engn & Technol, Tianjin 300072, Peoples R China
推荐引用方式 GB/T 7714	Li, Haitao,Li, Yuping,Cao, Hongbin,et al. Degradation of pentachlorophenol by a novel peroxidase-catalyzed process in the presence of reduced nicotinamide adenine dinucleotide[J]. CHEMOSPHERE,2011,83(2):124-130.
APA	Li, Haitao,Li, Yuping,Cao, Hongbin,Li, Xingang,&Zhang, Yi.(2011).Degradation of pentachlorophenol by a novel peroxidase-catalyzed process in the presence of reduced nicotinamide adenine dinucleotide.CHEMOSPHERE,83(2),124-130.
MLA	Li, Haitao,et al."Degradation of pentachlorophenol by a novel peroxidase-catalyzed process in the presence of reduced nicotinamide adenine dinucleotide".CHEMOSPHERE 83.2(2011):124-130.