Novel Inhibitor Cystine Knot Peptides from Momordica charantia

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	Novel Inhibitor Cystine Knot Peptides from Momordica charantia
	He, Wen-Jun 1; Chan, Lai Yue 2; Clark, Richard J.2,3; Tang, Jun 1; Zeng, Guang-Zhi 1; Franco, Octavio L.4; Cantacessi, Cinzia 5; Craik, David J.2; Daly, Norelle L.2,5; Tan, Ning-Hua 1
刊名	PLOS ONE
	2013-10-08
卷号	8 期号:10 页码:e75334
关键词	AMINO-ACID-SEQUENCES TRYPSIN-INHIBITOR PLASMODIUM-FALCIPARUM PROTEINASE-INHIBITORS CYTOTOXIC CYCLOTIDES MASS-SPECTROMETRY FOLDING PATHWAYS LINN SEEDS KALATA B1 X-RAY
ISSN号	1932-6203
通讯作者	Daly, NL (reprint author), Univ Queensland, Inst Mol Biosci, Brisbane, Qld, Australia.,norelle.daly@jcu.edu.au ; nhtan@mail.kib.ac.cn
英文摘要	Two new peptides, MCh-1 and MCh-2, along with three known trypsin inhibitors (MCTI-I, MCTI-II and MCTI-III), were isolated from the seeds of the tropical vine Momordica charantia. The sequences of the peptides were determined using mass spectrometry and NMR spectroscopy. Using a strategy involving partial reduction and stepwise alkylation of the peptides, followed by enzymatic digestion and tandem mass spectrometry sequencing, the disulfide connectivity of MCh-1 was elucidated to be CysI-CysIV, CysII-CysV and CysIII-CysVI. The three-dimensional structures of MCh-1 and MCh-2 were determined using NMR spectroscopy and found to contain the inhibitor cystine knot (ICK) motif. The sequences of the novel peptides differ significantly from peptides previously isolated from this plant. Therefore, this study expands the known peptide diversity in M. charantia and the range of sequences that can be accommodated by the ICK motif. Furthermore, we show that a stable two-disulfide intermediate is involved in the oxidative folding of MCh-1. This disulfide intermediate is structurally homologous to the proposed ancestral fold of ICK peptides, and provides a possible pathway for the evolution of this structural motif, which is highly prevalent in nature.
学科主题	Multidisciplinary Sciences
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	AMINO-ACID-SEQUENCES ; TRYPSIN-INHIBITOR ; PLASMODIUM-FALCIPARUM ; PROTEINASE-INHIBITORS ; CYTOTOXIC CYCLOTIDES ; MASS-SPECTROMETRY ; FOLDING PATHWAYS ; LINN SEEDS ; KALATA B1 ; X-RAY
收录类别	SCI
资助信息	National Basic Research Program of China [2013CB127505, 2009CB522300]; National New Drug Innovation Great Project of China [2011ZX09307-002-02]; National Natural Science Foundation of China [21202174, U1032602, 91013002, 30725048]; foundation of Chinese Academy of Sciences (Hundred Talents Program); Natural Science Foundation of Yunnan Province [2012GA003]; China Scholarship Council; Queensland Smart State Fellowship; Conselho Nacional de Desenvolvimento Cientifico e Tecnologico; Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior; Fundacao de Apoio a Pesquisa do Distrito Federal
语种	英语
WOS记录号	WOS:000325552200016
公开日期	2013-11-12
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/17278]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources West Ch, Kunming Inst Bot, Kunming, Yunnan, Peoples R China 2.Univ Queensland, Inst Mol Biosci, Brisbane, Qld, Australia 3.Univ Queensland, Sch Biomed Sci, Brisbane, Qld, Australia 4.Univ Catolica Brasilia, Ctr Anal Prote & Bioquim, Programa Posgrad Ciencias Genom & Biotecnol, Brasilia, DF, Brazil 5.James Cook Univ, Ctr Biodiscovery & Mol Dev Therapeut, Cairns, Qld, Australia
推荐引用方式 GB/T 7714	He, Wen-Jun,Chan, Lai Yue,Clark, Richard J.,et al. Novel Inhibitor Cystine Knot Peptides from Momordica charantia[J]. PLOS ONE,2013,8(10):e75334.
APA	He, Wen-Jun.,Chan, Lai Yue.,Clark, Richard J..,Tang, Jun.,Zeng, Guang-Zhi.,...&Tan, Ning-Hua.(2013).Novel Inhibitor Cystine Knot Peptides from Momordica charantia.PLOS ONE,8(10),e75334.
MLA	He, Wen-Jun,et al."Novel Inhibitor Cystine Knot Peptides from Momordica charantia".PLOS ONE 8.10(2013):e75334.