Target replacement strategy for selection of DNA aptamers against the Fc region of mouse IgG | |
Ma, J.1; Wang, M. G.1; Mao, A. H.2; Zeng, J. Y.3; Liu, Y. Q.2; Wang, X. Q.2; Ma, J.2; Tian, Y. J.4; Ma, N.4; Yang, N.3 | |
刊名 | GENETICS AND MOLECULAR RESEARCH
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2013 | |
卷号 | 12期号:2页码:1399-1410 |
关键词 | Target replacement DNA aptamer Fc region of mouse IgG SELEX |
ISSN号 | 1676-5680 |
DOI | 10.4238/2013.April.25.11 |
英文摘要 | Aptamers that recognize the IgG Fc region are of great interest because of their wide application as an immunology probing tool, for diagnostics, and as affinity agents for antibody purification. We developed a target replacement strategy as a modification of conventional Systematic Evolution of Ligands by EXponential enrichment (SELEX) in order to efficiently select and identify novel DNA aptamers against the Fc region of mouse IgG. In this new approach, multiple IgG subclasses (IgG1, IgG2a, mouse IgG Fc, and anti-HBs IgG) were sequentially used to select aptamers in one continuous SELEX. After 8 rounds of selection, the aptamers were analyzed using dot blot and an electrophoretic mobility shift assay, which showed universal binding capability to different IgG subclasses. Secondary structure analysis of the aptamers indicated that the stem-loop structure of the aptamers play an important role in binding to the common site in different mouse IgG subclasses. This demonstrated the feasibility of using multiple target replacement SELEX for the selection of aptamers. This target replacement strategy is also expected to be useful for selecting aptamers that bind common regions of molecules other than antibodies. |
资助项目 | Fund of Innovative Technology for Small or Medium-Sized Enterprises in China[06C26226200591] |
WOS研究方向 | Biochemistry & Molecular Biology ; Genetics & Heredity |
语种 | 英语 |
出版者 | FUNPEC-EDITORA |
WOS记录号 | WOS:000320030100054 |
状态 | 已发表 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.223/handle/2XXMBERH/34795] ![]() |
专题 | 兰州理工大学 |
通讯作者 | Liao, S. Q. |
作者单位 | 1.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou, Gansu, Peoples R China 2.Gansu Acad Med Sci, Lanzhou, Gansu, Peoples R China 3.Northwest Normal Univ, Coll Life Sci, Lanzhou, Gansu, Peoples R China 4.Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Gansu, Peoples R China |
推荐引用方式 GB/T 7714 | Ma, J.,Wang, M. G.,Mao, A. H.,et al. Target replacement strategy for selection of DNA aptamers against the Fc region of mouse IgG[J]. GENETICS AND MOLECULAR RESEARCH,2013,12(2):1399-1410. |
APA | Ma, J..,Wang, M. G..,Mao, A. H..,Zeng, J. Y..,Liu, Y. Q..,...&Liao, S. Q..(2013).Target replacement strategy for selection of DNA aptamers against the Fc region of mouse IgG.GENETICS AND MOLECULAR RESEARCH,12(2),1399-1410. |
MLA | Ma, J.,et al."Target replacement strategy for selection of DNA aptamers against the Fc region of mouse IgG".GENETICS AND MOLECULAR RESEARCH 12.2(2013):1399-1410. |
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