Characterization of ribose-5-phosphate isomerase converting D-psicose to D-allose from Thermotoga lettingae TMO | |
Feng, Zaiping1,2; Mu, Wanmeng1; Jiang, Bo1 | |
刊名 | BIOTECHNOLOGY LETTERS
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2013-05 | |
卷号 | 35期号:5页码:719-724 |
关键词 | D-Allose D-Psicose Ribose-5-phosphate isomerase Thermotoga lettingae |
ISSN号 | 0141-5492 |
DOI | 10.1007/s10529-013-1136-3 |
英文摘要 | The gene coding for ribose-5-phosphate isomerase (Rpi) from Thermotoga lettingae TMO was cloned and expressed in E. coli. The recombinant enzyme was purified by Ni-affinity chromatography. It converted d-psicose to d-allose maximally at 75 A degrees C and pH 8.0 with a 32 % conversion yield. The k (m), turnover number (k (cat)), and catalytic efficiency (k (cat) k (m) (-1) ) for substrate d-psicose were 64 mM, 6.98 min(-1) and 0.11 mM(-1) min(-1) respectively. |
WOS研究方向 | Biotechnology & Applied Microbiology |
语种 | 英语 |
出版者 | SPRINGER |
WOS记录号 | WOS:000318510500009 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.223/handle/2XXMBERH/34705] ![]() |
专题 | 兰州理工大学 |
作者单位 | 1.Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China; 2.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China |
推荐引用方式 GB/T 7714 | Feng, Zaiping,Mu, Wanmeng,Jiang, Bo. Characterization of ribose-5-phosphate isomerase converting D-psicose to D-allose from Thermotoga lettingae TMO[J]. BIOTECHNOLOGY LETTERS,2013,35(5):719-724. |
APA | Feng, Zaiping,Mu, Wanmeng,&Jiang, Bo.(2013).Characterization of ribose-5-phosphate isomerase converting D-psicose to D-allose from Thermotoga lettingae TMO.BIOTECHNOLOGY LETTERS,35(5),719-724. |
MLA | Feng, Zaiping,et al."Characterization of ribose-5-phosphate isomerase converting D-psicose to D-allose from Thermotoga lettingae TMO".BIOTECHNOLOGY LETTERS 35.5(2013):719-724. |
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