Characterization of ribose-5-phosphate isomerase converting D-psicose to D-allose from Thermotoga lettingae TMO

doi:10.1007/s10529-013-1136-3

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	Characterization of ribose-5-phosphate isomerase converting D-psicose to D-allose from Thermotoga lettingae TMO
	Feng, Zaiping 1,2; Mu, Wanmeng 1; Jiang, Bo 1
刊名	BIOTECHNOLOGY LETTERS
	2013-05
卷号	35 期号:5 页码:719-724
关键词	D-Allose D-Psicose Ribose-5-phosphate isomerase Thermotoga lettingae
ISSN号	0141-5492
DOI	10.1007/s10529-013-1136-3
英文摘要	The gene coding for ribose-5-phosphate isomerase (Rpi) from Thermotoga lettingae TMO was cloned and expressed in E. coli. The recombinant enzyme was purified by Ni-affinity chromatography. It converted d-psicose to d-allose maximally at 75 A degrees C and pH 8.0 with a 32 % conversion yield. The k (m), turnover number (k (cat)), and catalytic efficiency (k (cat) k (m) (-1) ) for substrate d-psicose were 64 mM, 6.98 min(-1) and 0.11 mM(-1) min(-1) respectively.
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000318510500009
内容类型	期刊论文
源URL	[http://119.78.100.223/handle/2XXMBERH/34705]
专题	兰州理工大学
作者单位	1.Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China; 2.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Peoples R China
推荐引用方式 GB/T 7714	Feng, Zaiping,Mu, Wanmeng,Jiang, Bo. Characterization of ribose-5-phosphate isomerase converting D-psicose to D-allose from Thermotoga lettingae TMO[J]. BIOTECHNOLOGY LETTERS,2013,35(5):719-724.
APA	Feng, Zaiping,Mu, Wanmeng,&Jiang, Bo.(2013).Characterization of ribose-5-phosphate isomerase converting D-psicose to D-allose from Thermotoga lettingae TMO.BIOTECHNOLOGY LETTERS,35(5),719-724.
MLA	Feng, Zaiping,et al."Characterization of ribose-5-phosphate isomerase converting D-psicose to D-allose from Thermotoga lettingae TMO".BIOTECHNOLOGY LETTERS 35.5(2013):719-724.