Molecular and expression characterization of a nanos1 homologue in Chinese sturgeon, Acipenser sinensis

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	Molecular and expression characterization of a nanos1 homologue in Chinese sturgeon, Acipenser sinensis
	Ye, Huan 1,4; Chen, Xihua 1; Wei, Qiwei 1,3; Zhou, Li 2; Liu, Tao 4; Gui, Jianfang 2; Li, Chuangju 1,3; Cao, Hong 2
刊名	GENE
	2012-12-15
卷号	511 期号:2 页码:285-292
关键词	Nanos1 Chinese sturgeon Expression pattern
ISSN号	0378-1119
通讯作者	Li, CJ (reprint author), Chinese Acad Fisheries Sci, Yangtze River Fisheries Res Inst, Minisny Agr China, Key Lab Freshwater Biodivers Conservat, Wuhan 430223, Peoples R China.
中文摘要	The nanos gene family was essential for germ line development in diverse organisms. In the present study, the full-length cDNA of a nanos1 homologue in A. sinensis, Asnanos1, was isolated and characterized. The cDNA sequence of Asnanos1 was 1489 base pairs (bp) in length and encoded a peptide of 228 amino acid residues. Multiple sequence alignment showed that the zinc-finger motifs of Nanos1 were highly conserved in vertebrates. By RT-PCR analysis, Asnanos1 mRNAs were ubiquitously detected in all tissues examined except for the fat, including liver, spleen, heart, ovary, kidney, muscle, intestines, pituitary, hypothalamus, telencephalon, midbrain, cerebellum, and medulla oblongata. Moreover, a specific polyclonal antibody was prepared from the in vitro expressed partial AsNanos1 protein. Western blot analysis revealed that the tissue expression pattern of AsNanos1 was not completely coincided with that of its mRNAs, which was not found in fat, muscle and intestines. Additionally, by immunofluoresence localization, it was observed that AsNanos1 protein was in the cytoplasm of primary oocytes and spermatocytes. The presented results indicated that the expression pattern of Asnanos1 was differential conservation and divergence among diverse species. (C) 2012 Elsevier B.V. All rights reserved.
英文摘要	The nanos gene family was essential for germ line development in diverse organisms. In the present study, the full-length cDNA of a nanos1 homologue in A. sinensis, Asnanos1, was isolated and characterized. The cDNA sequence of Asnanos1 was 1489 base pairs (bp) in length and encoded a peptide of 228 amino acid residues. Multiple sequence alignment showed that the zinc-finger motifs of Nanos1 were highly conserved in vertebrates. By RT-PCR analysis, Asnanos1 mRNAs were ubiquitously detected in all tissues examined except for the fat, including liver, spleen, heart, ovary, kidney, muscle, intestines, pituitary, hypothalamus, telencephalon, midbrain, cerebellum, and medulla oblongata. Moreover, a specific polyclonal antibody was prepared from the in vitro expressed partial AsNanos1 protein. Western blot analysis revealed that the tissue expression pattern of AsNanos1 was not completely coincided with that of its mRNAs, which was not found in fat, muscle and intestines. Additionally, by immunofluoresence localization, it was observed that AsNanos1 protein was in the cytoplasm of primary oocytes and spermatocytes. The presented results indicated that the expression pattern of Asnanos1 was differential conservation and divergence among diverse species. (C) 2012 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Genetics & Heredity
研究领域[WOS]	Genetics & Heredity
关键词[WOS]	GERM-CELL DEVELOPMENT ; CAENORHABDITIS-ELEGANS ; DENDRITE MORPHOGENESIS ; PLASM COMPONENT ; STEM-CELLS ; GENES ; RNA ; CONSERVATION ; POSTERIOR ; PROTEINS
收录类别	SCI
资助信息	National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences [2011JBFA22]; National Natural Science Foundation of China [31001104]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]
语种	英语
WOS记录号	WOS:000313024700021
公开日期	2013-10-31
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/19319]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Chinese Acad Fisheries Sci, Yangtze River Fisheries Res Inst, Minisny Agr China, Key Lab Freshwater Biodivers Conservat, Wuhan 430223, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 3.Chinese Acad Fisheries Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Peoples R China 4.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
推荐引用方式 GB/T 7714	Ye, Huan,Chen, Xihua,Wei, Qiwei,et al. Molecular and expression characterization of a nanos1 homologue in Chinese sturgeon, Acipenser sinensis[J]. GENE,2012,511(2):285-292.
APA	Ye, Huan.,Chen, Xihua.,Wei, Qiwei.,Zhou, Li.,Liu, Tao.,...&Cao, Hong.(2012).Molecular and expression characterization of a nanos1 homologue in Chinese sturgeon, Acipenser sinensis.GENE,511(2),285-292.
MLA	Ye, Huan,et al."Molecular and expression characterization of a nanos1 homologue in Chinese sturgeon, Acipenser sinensis".GENE 511.2(2012):285-292.