A Buckwheat (Fagopyrum esculentum) DRE-Binding Transcription Factor Gene, FeDREB1, Enhances Freezing and Drought Tolerance of Transgenic Arabidopsis

doi:10.1007/s11105-015-0851-4

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	A Buckwheat (Fagopyrum esculentum) DRE-Binding Transcription Factor Gene, FeDREB1, Enhances Freezing and Drought Tolerance of Transgenic Arabidopsis
	Fang, Zhengwu 2; Zhang, Xiaohong ; Gao, Jinfeng ; Wang, Pengke ; Xu, Xiaoyu ; Liu, Zhixiong 2; Shen, Shihua 3; Feng, Baili
刊名	PLANT MOLECULAR BIOLOGY REPORTER
	2015
卷号	33 期号:5 页码:1510-1525
关键词	Buckwheat CBF/DREB Abscisic acid (ABA) Freezing and drought tolerance
ISSN号	0735-9640
DOI	10.1007/s11105-015-0851-4
文献子类	Article
英文摘要	CBF/DREB transcription factors play essential roles in plant stress signaling transduction pathway. We isolated and identified a CBF/DREB homologous gene, FeDREB1, from Fagopyrum esculentum. Protein sequence alignment and phylogenetic analyses revealed that the FeDREB1 was grouped into the DREB (A-1) lineage. Moreover, subcellular localization observations suggested that FeDREB1 localizes in the nucleus. Yeast one-hybrid assays showed that FeDREB1 protein specifically binds to the DRE sequence and could activate the expression of reporter genes in yeast. These results further suggested that the FeRDEB1 protein was a CBF/DREB transcription factor. Expression analysis revealed that the transcript levels of the FeRDEB1 gene increased rapidly following low-/high-temperature treatment, drought stress, and exogenous ABA treatment. Over-expression of the FeDREB1 gene significantly enhanced the drought and freezing tolerance of transgenic Arabidopsis but resulted in its growth retardation. Moreover, the transgenic Arabidopsis lines were also highly sensitive to ABA application. Digital gene expression profiling (DGE) analysis indicated that increased transcript levels of many ABA-independent/-dependent stress-responsive genes in 35S::FeRDEB1 transgenic Arabidopsis, revealing that the FeDREB1 may participate in an ABA-dependent/-independent pathways.
学科主题	Biochemical Research Methods ; Plant Sciences
电子版国际标准刊号	1572-9818
出版地	NEW YORK
WOS关键词	COLD-RESPONSE PATHWAY ; LOW-TEMPERATURE ; FUNCTIONAL-ANALYSIS ; SALT TOLERANCE ; STRESS TOLERANCE ; ABSCISIC-ACID ; EXPRESSION ; DEHYDRATION ; RICE ; ELEMENT
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000360392800028
资助机构	National Nature Science Foundation of China [31071472] ; Special Fund for Agro-scientific Research in the Public Interest of China [201303008]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25627]
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100081, Peoples R China 2.Northwest A&F Univ, Coll Agron, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shanxi, Peoples R China 3.Yangtze Univ, Inst Crop Genet & Breeding, Coll Agr, Jingzhou 434025, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Fang, Zhengwu,Zhang, Xiaohong,Gao, Jinfeng,et al. A Buckwheat (Fagopyrum esculentum) DRE-Binding Transcription Factor Gene, FeDREB1, Enhances Freezing and Drought Tolerance of Transgenic Arabidopsis[J]. PLANT MOLECULAR BIOLOGY REPORTER,2015,33(5):1510-1525.
APA	Fang, Zhengwu.,Zhang, Xiaohong.,Gao, Jinfeng.,Wang, Pengke.,Xu, Xiaoyu.,...&Feng, Baili.(2015).A Buckwheat (Fagopyrum esculentum) DRE-Binding Transcription Factor Gene, FeDREB1, Enhances Freezing and Drought Tolerance of Transgenic Arabidopsis.PLANT MOLECULAR BIOLOGY REPORTER,33(5),1510-1525.
MLA	Fang, Zhengwu,et al."A Buckwheat (Fagopyrum esculentum) DRE-Binding Transcription Factor Gene, FeDREB1, Enhances Freezing and Drought Tolerance of Transgenic Arabidopsis".PLANT MOLECULAR BIOLOGY REPORTER 33.5(2015):1510-1525.