The NdhV subunit is required to stabilize the chloroplast NADH dehydrogenase-like complex in Arabidopsis | |
Fan, Xiangyuan1; Zhang, Jiao; Li, Wenjing; Peng, Lianwei | |
刊名 | PLANT JOURNAL |
2015 | |
卷号 | 82期号:2页码:221-231 |
关键词 | NADH dehydrogenase-like complex cyclic electron transport Arabidopsis chloroplast stabilization |
ISSN号 | 0960-7412 |
DOI | 10.1111/tpj.12807 |
文献子类 | Article |
英文摘要 | The chloroplast NADH dehydrogenase-like (NDH) complex is involved in cyclic electron transport around photosystemI (PSI) and chlororespiration. Although the NDH complex was discovered more than 20years ago, its low abundance and fragile nature render it recalcitrant to analysis, and it is thought that some of its subunits remain to be identified. Here, we identified the NDH subunit NdhV that readily disassociates from the NDH complex in the presence of detergent, salt and alkaline solutions. The Arabidopsis ndhv mutant is partially defective in the accumulation of NDH subcomplexA (SubA) and SubE, resulting in impaired NDH activity. NdhV was mainly detected in the wild-type thylakoid membrane, and its accumulation in thylakoids strictly depended on the presence of the NDH complex. Quantitative immunoblot analysis revealed that NdhV and NdhN occur at close to equimolar concentrations. Furthermore, several NDH subunits were co-immunopurified with NdhV using a combination of chemical crosslinking and an affinity chromatography assay. These data indicate that NdhV is an intrinsic subunit of NDH. We found that NdhV did not directly affect NDH activity, but that NDH SubA and SubE were more rapidly degraded in ndhv than in the wild type under high-light treatment. We propose that NdhV is an NDH subunit that stabilizes this complex, especially under high-light conditions. Significance Statement This work identifies the chloroplast NADH dehydrogenase-like (NDH) subunit NdhV, which is located on the most fragile part of the NDH complex. NdhV interacts with and stabilizes the NDH subcomplexes A and E, especially under high-light conditions. |
学科主题 | Plant Sciences |
电子版国际标准刊号 | 1365-313X |
出版地 | HOBOKEN |
WOS关键词 | CYCLIC ELECTRON FLOW ; DOMAIN-LIKE FOLD ; PHOTOSYSTEM-I ; NAD(P)H DEHYDROGENASE ; SUPERCOMPLEX FORMATION ; EFFICIENT OPERATION ; IDENTIFICATION ; OXIDOREDUCTASE ; PROTEINS ; PHOTOSYNTHESIS |
WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
语种 | 英语 |
出版者 | WILEY |
WOS记录号 | WOS:000352581600004 |
资助机构 | National Natural Science Foundation of China [31270289] ; Chinese Academy of Sciences |
内容类型 | 期刊论文 |
源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25703] |
专题 | 中科院光生物学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Fan, Xiangyuan,Zhang, Jiao,Li, Wenjing,et al. The NdhV subunit is required to stabilize the chloroplast NADH dehydrogenase-like complex in Arabidopsis[J]. PLANT JOURNAL,2015,82(2):221-231. |
APA | Fan, Xiangyuan,Zhang, Jiao,Li, Wenjing,&Peng, Lianwei.(2015).The NdhV subunit is required to stabilize the chloroplast NADH dehydrogenase-like complex in Arabidopsis.PLANT JOURNAL,82(2),221-231. |
MLA | Fan, Xiangyuan,et al."The NdhV subunit is required to stabilize the chloroplast NADH dehydrogenase-like complex in Arabidopsis".PLANT JOURNAL 82.2(2015):221-231. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论