Comparative proteomics of root plasma membrane proteins reveals the involvement of calcium signalling in NaCl-facilitated nitrate uptake in Salicornia europaea

doi:10.1093/jxb/erv216

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	Comparative proteomics of root plasma membrane proteins reveals the involvement of calcium signalling in NaCl-facilitated nitrate uptake in Salicornia europaea
	Nie, Lingling ; Feng, Juanjuan ; Fan, Pengxiang 1; Chen, Xianyang ; Guo, Jie ; Lv, Sulian ; Bao, Hexigeduleng 2; Jia, Weitao ; Tai, Fang ; Jiang, Ping
刊名	JOURNAL OF EXPERIMENTAL BOTANY
	2015
卷号	66 期号:15 页码:4497-4510
关键词	[Ca2+](cyt) calcium signalling 2D-DIGE NaCl nitrate uptake plasma membrane Salicornia europaea
ISSN号	0022-0957
DOI	10.1093/jxb/erv216
文献子类	Article
英文摘要	Improving crop nitrogen (N) use efficiency under salinity is essential for the development of sustainable agriculture in marginal lands. Salicornia europaea is a succulent euhalophyte that can survive under high salinity and N-deficient habitat conditions, implying that a special N assimilation mechanism may exist in this plant. In this study, phenotypic and physiological changes of S. europaea were investigated under different nitrate and NaCl levels. The results showed that NaCl had a synergetic effect with nitrate on the growth of S. europaea. In addition, the shoot nitrate concentration and nitrate uptake rate of S. europaea were increased by NaCl treatment under both low N and high N conditions, suggesting that nitrate uptake in S. europaea was NaCl facilitated. Comparative proteomic analysis of root plasma membrane (PM) proteins revealed 81 proteins, whose abundance changed significantly in response to NaCl and nitrate. These proteins are involved in metabolism, cell signalling, transport, protein folding, membrane trafficking, and cell structure. Among them, eight proteins were calcium signalling components, and the accumulation of seven of the above-mentioned proteins was significantly elevated by NaCl treatment. Furthermore, cytosolic Ca2+ concentration ([Ca2+](cyt)) was significantly elevated in S. europaea under NaCl treatment. The application of the Ca2+ channel blocker LaCl3 not only caused a decrease in nitrate uptake rate, but also attenuated the promoting effects of NaCl on nitrate uptake rates. Based on these results, a possible regulatory network of NaCl-facilitated nitrate uptake in S. europaea focusing on the involvement of Ca2+ signalling was proposed.
学科主题	Plant Sciences
电子版国际标准刊号	1460-2431
出版地	OXFORD
WOS关键词	STRESS-RESPONSIVE PROTEINS ; ARABIDOPSIS-THALIANA ; CYTOSOLIC CALCIUM ; NITROGEN NUTRITION ; SALT TOLERANCE ; SODIUM ; SALINITY ; PLANTS ; GROWTH ; TRANSPORT
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000359687400006
资助机构	Chinese Ministry of Agriculture [2014ZX08009-003-002] ; National Natural Science Foundation of China [31200201] ; Science and Technology Service Network Initiative of the Chinese Academy of Sciences [KFJ-EW-STS-061-4]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25810]
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Michigan State Univ, Dept Biochem & Mol Biol, E Lansing, MI 48824 USA 3.Chinese Acad Sci, Shanghai Ctr Plant Stress Biol PSC, Shanghai 201602, Peoples R China
推荐引用方式 GB/T 7714	Nie, Lingling,Feng, Juanjuan,Fan, Pengxiang,et al. Comparative proteomics of root plasma membrane proteins reveals the involvement of calcium signalling in NaCl-facilitated nitrate uptake in Salicornia europaea[J]. JOURNAL OF EXPERIMENTAL BOTANY,2015,66(15):4497-4510.
APA	Nie, Lingling.,Feng, Juanjuan.,Fan, Pengxiang.,Chen, Xianyang.,Guo, Jie.,...&Li, Yinxin.(2015).Comparative proteomics of root plasma membrane proteins reveals the involvement of calcium signalling in NaCl-facilitated nitrate uptake in Salicornia europaea.JOURNAL OF EXPERIMENTAL BOTANY,66(15),4497-4510.
MLA	Nie, Lingling,et al."Comparative proteomics of root plasma membrane proteins reveals the involvement of calcium signalling in NaCl-facilitated nitrate uptake in Salicornia europaea".JOURNAL OF EXPERIMENTAL BOTANY 66.15(2015):4497-4510.