Functional Divergence of Diterpene Syntheses in the Medicinal Plant Salvia miltiorrhiza

doi:10.1104/pp.15.00695

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院植物分子生理学重点实验室

	Functional Divergence of Diterpene Syntheses in the Medicinal Plant Salvia miltiorrhiza
	Cui, Guanghong 1; Duan, Lixin ; Jin, Baolong 1; Qian, Jun 4,5; Xue, Zheyong ; Shen, Guoan ; Snyder, John Hugh ; Song, Jingyuan 4,5; Chen, Shilin 4,5; Huang, Luqi 1
刊名	PLANT PHYSIOLOGY
	2015
卷号	169 期号:3 页码:1607-1618
ISSN号	0032-0889
DOI	10.1104/pp.15.00695
文献子类	Article
英文摘要	The medicinal plant Salvia miltiorrhiza produces various tanshinone diterpenoids that have pharmacological activities such as vasorelaxation against ischemia reperfusion injury and antiarrhythmic effects. Their biosynthesis is initiated from the general diterpenoid precursor (E,E,E)-geranylgeranyl diphosphate by sequential reactions catalyzed by copalyl diphosphate synthase (CPS) and kaurene synthase-like cyclases. Here, we report characterization of these enzymatic families from S. miltiorrhiza, which has led to the identification of unique pathways, including roles for separate CPSs in tanshinone production in roots versus aerial tissues (SmCPS1 and SmCPS2, respectively) as well as the unique production of ent-13-epi-manoyl oxide by SmCPS4 and S. miltiorrhiza kaurene synthase-like2 in floral sepals. The conserved SmCPS5 is involved in gibberellin plant hormone biosynthesis. Down-regulation of SmCPS1 by RNA interference resulted in substantial reduction of tanshinones, and metabolomics analysis revealed 21 potential intermediates, indicating a complex network for tanshinone metabolism defined by certain key biosynthetic steps. Notably, the correlation between conservation pattern and stereochemical product outcome of the CPSs observed here suggests a degree of correlation that, especially when combined with the identity of certain key residues, may be predictive. Accordingly, this study provides molecular insights into the evolutionary diversification of functional diterpenoids in plants.
学科主题	Plant Sciences
电子版国际标准刊号	1532-2548
出版地	ROCKVILLE
WOS关键词	SINGLE RESIDUE SWITCH ; COPALYL DIPHOSPHATE SYNTHASE ; POSITIVE SELECTION ; ABIETADIENE SYNTHASE ; MAXIMUM-LIKELIHOOD ; HAIRY ROOTS ; BIOSYNTHESIS ; TANSHINONES ; METABOLISM ; CHEMISTRY
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	AMER SOC PLANT BIOLOGISTS
WOS记录号	WOS:000367393900017
资助机构	Key Project of Chinese National Programs for Fundamental Research and Development [2013CB127000] ; National Science Fund for Distinguished Young Scholars [81325023] ; National Natural Science Foundation of China [81001604] ; China Academy of Chinese Medical Sciences [ZZ20090301] ; National Institutes of Health [GM076324] ; NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM076324] Funding Source: NIH RePORTER
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25642]
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Peters, Reuben J.] Iowa State Univ, Dept Biochem Biophys & Mol Biol, Ames, IA 50011 USA 3.Peking Union Med Coll, Beijing 100193, Peoples R China 4.Chinese Acad Med Sci, Inst Med Plant Dev, Beijing 100193, Peoples R China 5.China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, State Key Lab Breeding Base Daodi Herbs, Beijing 100700, Peoples R China
推荐引用方式 GB/T 7714	Cui, Guanghong,Duan, Lixin,Jin, Baolong,et al. Functional Divergence of Diterpene Syntheses in the Medicinal Plant Salvia miltiorrhiza[J]. PLANT PHYSIOLOGY,2015,169(3):1607-1618.
APA	Cui, Guanghong.,Duan, Lixin.,Jin, Baolong.,Qian, Jun.,Xue, Zheyong.,...&Qi, Xiaoquan.(2015).Functional Divergence of Diterpene Syntheses in the Medicinal Plant Salvia miltiorrhiza.PLANT PHYSIOLOGY,169(3),1607-1618.
MLA	Cui, Guanghong,et al."Functional Divergence of Diterpene Syntheses in the Medicinal Plant Salvia miltiorrhiza".PLANT PHYSIOLOGY 169.3(2015):1607-1618.