ycf1, the most promising plastid DNA barcode of land plants

doi:10.1038/srep08348

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	ycf1, the most promising plastid DNA barcode of land plants
	Dong, Wenpan ; Xu, Chao ; Li, Changhao 3; Sun, Jiahui 3; Zuo, Yunjuan ; Shi, Shuo ; Cheng, Tao ; Guo, Junjie 2; Zhou, Shiliang
刊名	SCIENTIFIC REPORTS
	2015
卷号	5
ISSN号	2045-2322
DOI	10.1038/srep08348
文献子类	Article
英文摘要	A DNA barcode is a DNA fragment used to identify species. For land plants, DNA fragments of plastid genome could be the primary consideration. Unfortunately, most of the plastid candidate barcodes lack species-level resolution. The identification of DNA barcodes of high resolution at species level is critical to the success of DNA barcoding in plants. We searched the available plastid genomes for the most variable regions and tested the best candidates using both a large number of tree species and seven well-sampled plant groups. Two regions of the plastid gene ycf1, ycf1a and ycf1b, were the most variable loci that were better than existing plastid candidate barcodes and can serve as a barcode of land plants. Primers were designed for the amplification of these regions, and the PCR success of these primers ranged from 82.80% to 98.17%. Of 420 tree species, 357 species could be distinguished using ycf1b, which was slightly better than the combination of matK and rbcL. For the well-sampled representative plant groups, ycf1b generally performed better than any of the matK, rbcL and trnH-psbA. We concluded that ycf1a or ycf1b is the most variable plastid genome region and can serve as a core barcode of land plants.
学科主题	Multidisciplinary Sciences
出版地	BERLIN
WOS关键词	CHLOROPLAST GENOME ; MATK PRIMERS ; PHYLOGENY ; GENE ; BIOGEOGRAPHY ; LEPECHINIA ; EVOLUTION ; RADIATION ; CLADE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	NATURE PORTFOLIO
WOS记录号	WOS:000349245100001
资助机构	Ministry of Science and Technology of China [2011FY120200, 2012BAC01B05, 2012AA021602] ; National Natural Science Foundation of China [31270239]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/26021]
专题	系统与进化植物学国家重点实验室
作者单位	1.Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Guangdong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Dong, Wenpan,Xu, Chao,Li, Changhao,et al. ycf1, the most promising plastid DNA barcode of land plants[J]. SCIENTIFIC REPORTS,2015,5.
APA	Dong, Wenpan.,Xu, Chao.,Li, Changhao.,Sun, Jiahui.,Zuo, Yunjuan.,...&Zhou, Shiliang.(2015).ycf1, the most promising plastid DNA barcode of land plants.SCIENTIFIC REPORTS,5.
MLA	Dong, Wenpan,et al."ycf1, the most promising plastid DNA barcode of land plants".SCIENTIFIC REPORTS 5(2015).