Novel fluorescent GLUT1 inhibitor for precision detection and fluorescence image-guided surgery in oral squamous cell carcinoma

doi:10.1002/ijc.34049

CORC > 自动化研究所 > 中国科学院自动化研究所 > 中国科学院分子影像重点实验室

	Novel fluorescent GLUT1 inhibitor for precision detection and fluorescence image-guided surgery in oral squamous cell carcinoma
	Tian, Yu 7,8,9; Tang, Chu 6; Shi, Guangyuan 5; Wang, Guorong 4; Du, Yang 2,3,7; Tian, Jie 1,2,3,6,7; Zhang, Haizhong 8
刊名	INTERNATIONAL JOURNAL OF CANCER
	2022-08-01
卷号	151 期号:3 页码:450-462
关键词	fluorescence molecular imaging glucose transporter type 1 image-guided surgery mouth neoplasms WZB117
ISSN号	0020-7136
DOI	10.1002/ijc.34049
通讯作者	Du, Yang(yang.du@ia.ac.cn) ; Tian, Jie(jie.tian@ia.ac.cn) ; Zhang, Haizhong(zhanghaizhong@301hospital.com.cn)
英文摘要	Early detection and complete resection of oral squamous cell carcinoma (OSCC) are crucial to improving patient survival and prognosis. However, specifically targeted imaging probes for OSCC detection are limited. Our study aimed to synthesize a novel near-infrared fluorescence (NIRF) probe for precision detection and fluorescence image-guided surgery in OSCC. Bioinformatics data indicated that glucose transporter 1 (GLUT1) is highly expressed in patients with OSCC. We demonstrated high and specific GLUT1 expression upon immunohistochemical staining of samples from 20 patients with OSCC. The specific expression of GLUT1 was further validated in both human OSCC cell lines and OSCC tumor xenografts. Based on these findings, the GLUT1 inhibitor WZB117 was utilized to synthesize a novel NIRF imaging probe, WZB117-IR820. The fluorescence molecular imaging data revealed that WZB117-IR820 could specifically bind to the tumor areas in an orthotopic OSCC mouse model after intravenous injection and could be further applied for precision fluorescence image-guided surgery with no residual tumor in the orthotopic CAL27-fLUC mouse tumor model. For further clinical translational application in patients with OSCC, precise delineation of OSCC tumor areas was achieved after topical application of the WZB117-IR820 imaging probe and was validated by histopathological and immunohistochemical analyses. In conclusion, we synthesized a novel fluorescent imaging probe, WZB117-IR820, which has potential clinical applications for early detection and fluorescence image-guided surgery in OSCC with no observable toxicity.
资助项目	Beijing Natural Science Foundation[7212207] ; Ministry of Science and Technology of China[2017YFA0205200] ; Ministry of Science and Technology of China[2017YFA0700401] ; China National Key Research and Development Plan Project[2017YFB1304300] ; National Natural Science Foundation of China[62027901] ; National Natural Science Foundation of China[81227901] ; National Natural Science Foundation of China[81470083] ; National Natural Science Foundation of China[81527805] ; National Natural Science Foundation of China[81871514] ; Subsidiary of PLA Major Project[AWS17J004]
WOS关键词	SURGICAL NAVIGATION ; CANCER ; HEAD ; MARGINS ; TRANSPORT ; VALIDATION ; SURVIVAL ; BINDING ; FAMILY ; SAFETY
WOS研究方向	Oncology
语种	英语
出版者	WILEY
WOS记录号	WOS:000795148100001
资助机构	Beijing Natural Science Foundation ; Ministry of Science and Technology of China ; China National Key Research and Development Plan Project ; National Natural Science Foundation of China ; Subsidiary of PLA Major Project
内容类型	期刊论文
源URL	[http://ir.ia.ac.cn/handle/173211/49407]
专题	自动化研究所_中国科学院分子影像重点实验室
通讯作者	Du, Yang; Tian, Jie; Zhang, Haizhong
作者单位	1.Beihang Univ, Beijing Adv Innovat Ctr Big Data Based Precis Med, Sch Med, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Beijing Key Lab Mol Imaging, Beijing, Peoples R China 4.Peking Union Med Coll Hosp, Peking Union Med Coll & Chinese Acad Med Sci, Dept Radiol, Beijing, Peoples R China 5.Univ Sci & Technol China, Sch Informat Sci & Technol, Hefei, Peoples R China 6.Xidian Univ, Engn Res Ctr Mol & Neuro Imaging, Sch Life Sci & Technol, Minist Educ, Xian, Peoples R China 7.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, State Key Lab Management & Control Complex Syst, Beijing, Peoples R China 8.Chinese Peoples Liberat Army Gen Hosp, Med Ctr 1, Dept Stomatol, 28 Fuxing Rd, Beijing, Peoples R China 9.Med Sch Chinese PLA, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Tian, Yu,Tang, Chu,Shi, Guangyuan,et al. Novel fluorescent GLUT1 inhibitor for precision detection and fluorescence image-guided surgery in oral squamous cell carcinoma[J]. INTERNATIONAL JOURNAL OF CANCER,2022,151(3):450-462.
APA	Tian, Yu.,Tang, Chu.,Shi, Guangyuan.,Wang, Guorong.,Du, Yang.,...&Zhang, Haizhong.(2022).Novel fluorescent GLUT1 inhibitor for precision detection and fluorescence image-guided surgery in oral squamous cell carcinoma.INTERNATIONAL JOURNAL OF CANCER,151(3),450-462.
MLA	Tian, Yu,et al."Novel fluorescent GLUT1 inhibitor for precision detection and fluorescence image-guided surgery in oral squamous cell carcinoma".INTERNATIONAL JOURNAL OF CANCER 151.3(2022):450-462.