Target-modulated competitive binding and exonuclease I-powered strategy for the simultaneous and rapid detection of biological targets

doi:10.1016/j.bios.2021.113817

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	Target-modulated competitive binding and exonuclease I-powered strategy for the simultaneous and rapid detection of biological targets
	Wang, Yingwen 1,2,3; Zhang, Dun 1,2,3; Zeng, Yan 1,2; Qi, Peng 1,2,3
刊名	BIOSENSORS & BIOELECTRONICS
	2022-02-15
卷号	198 页码:8
关键词	Simultaneous multiple-target detection UiO-67 Exonuclease I-Powered signal molecule release ATP cyt c
ISSN号	0956-5663
DOI	10.1016/j.bios.2021.113817
通讯作者	Zhang, Dun(zhangdun@qdio.ac.cn) ; Qi, Peng(qipeng@qdio.ac.cn)
英文摘要	Simultaneous multiple-target detection is essential for the prevention, identification, and treatment of numerous diseases. In this study, a novel strategy based on target-modulated competitive binding and exonuclease I (Exo I) powered signal molecule release was established with the advantages of rapid response and high selectivity and sensitivity. The strategy holds substantial potential for the development of versatile platforms for the simultaneous detection of biological targets. To mitigate the low load capacity and time-consuming responsive process of the Zr-MOF system, UiO-67 was chosen to replace UiO-66 (a typical Zr-MOF) as the nanocarrier for encapsulating more signal molecules, whereby the assembled double-stranded DNA (dsDNA) structures of UiO-67 acted as gatekeepers to form dsDNA-functionalized MOFs. Additionally, Exo I was introduced into the system to accelerate the release of the signal molecules. In the presence of biological targets, the competitive binding between the targets and aptamers caused the hydrolysis of the free DNA sequence by Exo I, promoting the release of signal molecules and leading to a rapid and significant increase in the fluorescence intensity. For adenosine triphosphate (ATP) and cytochrome c (cyt c), which were chosen as model biological targets, this sensor displayed detection limits as low as 5.03 and 6.11 fM, respectively. Moreover, the developed biosensor was successfully applied to the simultaneous detection of ATP and cyt c in spiked serum samples. Therefore, this strategy provides guidance for further research of biosensors for simultaneous multiple-target detection and propels the application of MOF carriers in biomedicine.
资助项目	National Natural Science Foundation of China[41876101] ; National Natural Science Foundation of China[41906037] ; Nantong Scientific Plan Foundation[JC2021054] ; Nantong Scientific Plan Foundation[2020NT04]
WOS研究方向	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
语种	英语
出版者	ELSEVIER ADVANCED TECHNOLOGY
WOS记录号	WOS:000781104400008
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/178819]
专题	海洋研究所_海洋腐蚀与防护研究发展中心
通讯作者	Zhang, Dun; Qi, Peng
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yingwen,Zhang, Dun,Zeng, Yan,et al. Target-modulated competitive binding and exonuclease I-powered strategy for the simultaneous and rapid detection of biological targets[J]. BIOSENSORS & BIOELECTRONICS,2022,198:8.
APA	Wang, Yingwen,Zhang, Dun,Zeng, Yan,&Qi, Peng.(2022).Target-modulated competitive binding and exonuclease I-powered strategy for the simultaneous and rapid detection of biological targets.BIOSENSORS & BIOELECTRONICS,198,8.
MLA	Wang, Yingwen,et al."Target-modulated competitive binding and exonuclease I-powered strategy for the simultaneous and rapid detection of biological targets".BIOSENSORS & BIOELECTRONICS 198(2022):8.