Transcriptome Analysis of the Nematodes Caenorhabditis elegans and Litoditis marina in Different Food Environments

doi:10.3390/jmse10050580

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Transcriptome Analysis of the Nematodes Caenorhabditis elegans and Litoditis marina in Different Food Environments
	Sun, Peiqi 1,2,3,4; Cao, Xuwen 1,2,3,4; Zhang, Liusuo 1,3,4
刊名	JOURNAL OF MARINE SCIENCE AND ENGINEERING
	2022-05-01
卷号	10 期号:5 页码:18
关键词	C. elegans L. marina lifespan development FOXO DAF-16 lysosome
DOI	10.3390/jmse10050580
通讯作者	Zhang, Liusuo(lzhang@qdio.ac.cn)
英文摘要	Diets regulate animal development, reproduction, and lifespan. However, the underlying molecular mechanisms remain elusive. We previously showed that a chemically defined CeMM diet attenuates the development and promotes the longevity of C. elegans, but whether it impacts other nematodes is unknown. Here, we studied the effects of the CeMM diet on the development and longevity of the marine nematode Litoditis marina, which belongs to the same family as C. elegans. We further investigated genome-wide transcriptional responses to the CeMM and OP50 diets for both nematodes, respectively. We observed that the CeMM diet attenuated L. marina development but did not extend its lifespan. Through KEEG enrichment analysis, we found that many of the FOXO DAF-16 signaling and lysosome and xenobiotic metabolism related genes were significantly increased in C. elegans on the CeMM diet, which might contribute to the lifespan extension of C. elegans. Notably, we found that the expression of lysosome and xenobiotic metabolism pathway genes was significantly down-regulated in L. marina on CeMM, which might explain why the CeMM diet could not promote the lifespan of L. marina compared to bacterial feeding. Additionally, the down-regulation of several RNA transcription and protein generation and related processes genes in C. elegans on CeMM might not only be involved in extending longevity, but also contribute to attenuating the development of C. elegans on the CeMM diet, while the down-regulation of unsaturated fatty acids synthesis genes in L. marina might contribute to slow down its growth while on CeMM. This study provided important insights into how different diets regulate development and lifespan, and further genetic analysis of the candidate gene(s) of development and longevity will facilitate exploring the molecular mechanisms underlying how diets regulate animal physiology and health in the context of variable nutritional environments.
资助项目	National Key R and D Program of China[2018YFD0901301] ; Talents from overseas Program, IOCAS of the Chinese Academy of Sciences ; Qingdao Innovation Leadership Program[16-8-3-19-zhc] ; Key deployment project of the Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences[COMS2019Q16]
WOS研究方向	Engineering ; Oceanography
语种	英语
出版者	MDPI
WOS记录号	WOS:000801679000001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/179362]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Zhang, Liusuo
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nankai Rd, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Sun, Peiqi,Cao, Xuwen,Zhang, Liusuo. Transcriptome Analysis of the Nematodes Caenorhabditis elegans and Litoditis marina in Different Food Environments[J]. JOURNAL OF MARINE SCIENCE AND ENGINEERING,2022,10(5):18.
APA	Sun, Peiqi,Cao, Xuwen,&Zhang, Liusuo.(2022).Transcriptome Analysis of the Nematodes Caenorhabditis elegans and Litoditis marina in Different Food Environments.JOURNAL OF MARINE SCIENCE AND ENGINEERING,10(5),18.
MLA	Sun, Peiqi,et al."Transcriptome Analysis of the Nematodes Caenorhabditis elegans and Litoditis marina in Different Food Environments".JOURNAL OF MARINE SCIENCE AND ENGINEERING 10.5(2022):18.