CRISPR/Cas9-mediated efficient targeted mutagenesis in Chardonnay (Vitis vinifera L.)

doi:10.1038/srep32289

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院北方资源植物重点实验室

	CRISPR/Cas9-mediated efficient targeted mutagenesis in Chardonnay (Vitis vinifera L.)
	Ren, Chong 1,4; Liu, Xianju 1,4; Zhang, Zhan 1,4; Wang, Yi 1,4; Duan, Wei 4; Li, Shaohua 4; Liang, Zhenchang 3,4
刊名	SCIENTIFIC REPORTS
	2016
卷号	6
ISSN号	2045-2322
DOI	10.1038/srep32289
文献子类	Article
英文摘要	The type II clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 system (CRISPR/Cas9) has been successfully applied to edit target genes in multiple plant species. However, it remains unknown whether this system can be used for genome editing in grape. In this study, we described genome editing and targeted gene mutation in 'Chardonnay' suspension cells and plants via the CRISPR/Cas9 system. Two single guide RNAs (sgRNAs) were designed to target distinct sites of the L-idonate dehydrogenase gene (IdnDH). CEL I endonuclease assay and sequencing results revealed the expected indel mutations at the target site, and a mutation frequency of 100% was observed in the transgenic cell mass (CM) as well as corresponding regenerated plants with expression of sgRNA1/Cas9. The majority of the detected mutations in transgenic CM were 1-bp insertions, followed by 1-to 3-nucleotide deletions. Off-target activities were also evaluated by sequencing the potential off-target sites, and no obvious off-target events were detected. Our results demonstrated that the CRISPR/Cas9 system is an efficient and specific tool for precise genome editing in grape.
学科主题	Multidisciplinary Sciences
出版地	LONDON
WOS关键词	CRISPR-CAS SYSTEM ; RNA-GUIDED ENDONUCLEASE ; GENETIC-TRANSFORMATION ; GENOME MODIFICATIONS ; TRANSGENIC PLANTS ; LATER GENERATIONS ; ANALYSIS REVEALS ; 1ST GENERATION ; NUCLEASES ; RICE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000382239600001
资助机构	National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31572090] ; Agricultural Breeding Project of Ningxia Hui Autonomous Region [NXNYYZ20150203] ; Hundred Talents of Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25284]
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Plant Resource, Inst Bot, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Sino Africa Joint Res Ctr, Wuhan 430074, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Beijing Key Lab Grape Sci & Enol, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Ren, Chong,Liu, Xianju,Zhang, Zhan,et al. CRISPR/Cas9-mediated efficient targeted mutagenesis in Chardonnay (Vitis vinifera L.)[J]. SCIENTIFIC REPORTS,2016,6.
APA	Ren, Chong.,Liu, Xianju.,Zhang, Zhan.,Wang, Yi.,Duan, Wei.,...&Liang, Zhenchang.(2016).CRISPR/Cas9-mediated efficient targeted mutagenesis in Chardonnay (Vitis vinifera L.).SCIENTIFIC REPORTS,6.
MLA	Ren, Chong,et al."CRISPR/Cas9-mediated efficient targeted mutagenesis in Chardonnay (Vitis vinifera L.)".SCIENTIFIC REPORTS 6(2016).