CRISPR/Cas9-mediated efficient targeted mutagenesis in Chardonnay (Vitis vinifera L.) | |
Ren, Chong1,4; Liu, Xianju1,4; Zhang, Zhan1,4; Wang, Yi1,4; Duan, Wei4; Li, Shaohua4; Liang, Zhenchang3,4 | |
刊名 | SCIENTIFIC REPORTS
![]() |
2016 | |
卷号 | 6 |
ISSN号 | 2045-2322 |
DOI | 10.1038/srep32289 |
文献子类 | Article |
英文摘要 | The type II clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 system (CRISPR/Cas9) has been successfully applied to edit target genes in multiple plant species. However, it remains unknown whether this system can be used for genome editing in grape. In this study, we described genome editing and targeted gene mutation in 'Chardonnay' suspension cells and plants via the CRISPR/Cas9 system. Two single guide RNAs (sgRNAs) were designed to target distinct sites of the L-idonate dehydrogenase gene (IdnDH). CEL I endonuclease assay and sequencing results revealed the expected indel mutations at the target site, and a mutation frequency of 100% was observed in the transgenic cell mass (CM) as well as corresponding regenerated plants with expression of sgRNA1/Cas9. The majority of the detected mutations in transgenic CM were 1-bp insertions, followed by 1-to 3-nucleotide deletions. Off-target activities were also evaluated by sequencing the potential off-target sites, and no obvious off-target events were detected. Our results demonstrated that the CRISPR/Cas9 system is an efficient and specific tool for precise genome editing in grape. |
学科主题 | Multidisciplinary Sciences |
出版地 | LONDON |
WOS关键词 | CRISPR-CAS SYSTEM ; RNA-GUIDED ENDONUCLEASE ; GENETIC-TRANSFORMATION ; GENOME MODIFICATIONS ; TRANSGENIC PLANTS ; LATER GENERATIONS ; ANALYSIS REVEALS ; 1ST GENERATION ; NUCLEASES ; RICE |
WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
语种 | 英语 |
出版者 | NATURE PUBLISHING GROUP |
WOS记录号 | WOS:000382239600001 |
资助机构 | National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31572090] ; Agricultural Breeding Project of Ningxia Hui Autonomous Region [NXNYYZ20150203] ; Hundred Talents of Chinese Academy of Sciences |
内容类型 | 期刊论文 |
源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25284] ![]() |
专题 | 中科院北方资源植物重点实验室 |
作者单位 | 1.Chinese Acad Sci, Key Lab Plant Resource, Inst Bot, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Sino Africa Joint Res Ctr, Wuhan 430074, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Beijing Key Lab Grape Sci & Enol, Inst Bot, Beijing 100093, Peoples R China |
推荐引用方式 GB/T 7714 | Ren, Chong,Liu, Xianju,Zhang, Zhan,et al. CRISPR/Cas9-mediated efficient targeted mutagenesis in Chardonnay (Vitis vinifera L.)[J]. SCIENTIFIC REPORTS,2016,6. |
APA | Ren, Chong.,Liu, Xianju.,Zhang, Zhan.,Wang, Yi.,Duan, Wei.,...&Liang, Zhenchang.(2016).CRISPR/Cas9-mediated efficient targeted mutagenesis in Chardonnay (Vitis vinifera L.).SCIENTIFIC REPORTS,6. |
MLA | Ren, Chong,et al."CRISPR/Cas9-mediated efficient targeted mutagenesis in Chardonnay (Vitis vinifera L.)".SCIENTIFIC REPORTS 6(2016). |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论