Crystal structure of glutamate-1-semialdehyde-2,1-aminomutase from Arabidopsis thaliana | |
Song, Yingxian; Pu, Hua; Jiang, Tian; Zhang, Lixin; Ouyang, Min | |
刊名 | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS |
2016 | |
卷号 | 72页码:448-456 |
关键词 | X-ray crystallography asymmetry pyridoxamine 5 '-phosphate pyridoxal 5 '-phosphate gating loop glutamate-1-semialdehyde-2,1-aminomutase Arabidopsis thaliana |
DOI | 10.1107/S2053230X16007263 |
文献子类 | Article |
英文摘要 | Glutamate-1-semialdehyde-2,1-aminomutase (GSAM) catalyzes the isomerization of glutamate-1-semialdehyde (GSA) to 5-aminolevulinate (ALA) and is distributed in archaea, most bacteria and plants. Although structures of GSAM from archaea and bacteria have been resolved, a GSAM structure from a higher plant is not available, preventing further structure-function analysis. Here, the structure of GSAM from Arabidopsis thaliana (AtGSA1) obtained by X-ray crystallography is reported at 1.25 angstrom resolution. AtGSA1 forms an asymmetric dimer and displays asymmetry in cofactor binding as well as in the gating-loop orientation, which is consistent with previously reported Synechococcus GSAM structures. While one monomer binds PMP with the gating loop fixed in the open state, the other monomer binds either PMP or PLP and the gating loop is ready to close. The data also reveal the mobility of residues Gly163, Ser164 and Gly165, which are important for reorientation of the gating loop. Furthermore, the asymmetry of the AtGSA1 structure supports the previously proposed negative cooperativity between monomers of GSAM. |
学科主题 | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography |
电子版国际标准刊号 | 2053-230X |
出版地 | CHESTER |
WOS关键词 | GLUTAMYL-TRANSFER-RNA ; NEGATIVE COOPERATIVITY ; 1-SEMIALDEHYDE AMINOTRANSFERASE ; SEMIALDEHYDE AMINOTRANSFERASE ; CHLOROPHYLL BIOSYNTHESIS ; ENZYME ; 2,1-AMINOMUTASE ; SYNECHOCOCCUS ; MECHANISM ; REDUCTASE |
WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
语种 | 英语 |
出版者 | INT UNION CRYSTALLOGRAPHY |
WOS记录号 | WOS:000380288600005 |
资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31471267] |
内容类型 | 期刊论文 |
源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25313] |
专题 | 中科院光生物学重点实验室 |
作者单位 | Chinese Acad Sci, Photosynth Res Ctr, Key Lab Photobiol, Inst Bot, Beijing 100093, Peoples R China |
推荐引用方式 GB/T 7714 | Song, Yingxian,Pu, Hua,Jiang, Tian,et al. Crystal structure of glutamate-1-semialdehyde-2,1-aminomutase from Arabidopsis thaliana[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,2016,72:448-456. |
APA | Song, Yingxian,Pu, Hua,Jiang, Tian,Zhang, Lixin,&Ouyang, Min.(2016).Crystal structure of glutamate-1-semialdehyde-2,1-aminomutase from Arabidopsis thaliana.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,72,448-456. |
MLA | Song, Yingxian,et al."Crystal structure of glutamate-1-semialdehyde-2,1-aminomutase from Arabidopsis thaliana".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 72(2016):448-456. |
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