Glutathione reductase 2 maintains the function of photosystem II in Arabidopsis under excess light

doi:10.1016/j.bbabio.2016.02.011

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	Glutathione reductase 2 maintains the function of photosystem II in Arabidopsis under excess light
	Ding, Shunhua ; Jiang, Rui 2; Lu, Qingtao ; Wen, Xiaogang ; Lu, Congming
刊名	BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
	2016
卷号	1857 期号:6 页码:665-677
关键词	Arabidopsis Excess light Glutathione reductase 2 Photoinhibition Photosystem II
ISSN号	0005-2728
DOI	10.1016/j.bbabio.2016.02.011
文献子类	Article
英文摘要	Glutathione reductase plays a crucial role in the elimination of H2O2 molecules via the ascorbate-glutathione cycle. In this study, we used transgenic Arabidopsis plants with decreased glutathione reductase 2 (GR2) levels to investigate whether this GR2 activity protects the photosynthetic machinery under excess light. The transgenic plants were highly sensitive to excess light and accumulated high levels of H2O2. Photosystem II (PSII) activity was significantly decreased in transgenic plants. Flash-induced fluorescence relaxation and thermoluminescence measurements demonstrated inhibition of electron transfer between Q(A) and Q(B) and decreased redox potential of Q(B) in transgenic plants. Immunoblot and blue native gel analysis showed that the levels of PSII proteins and PSII complexes were decreased in transgenic plants. Analyses of the repair of photodamaged PSII and in vivo pulse labeling of thylakoid proteins showed that the repair of photodamaged PSII is inhibited due to the inhibition of the synthesis of the D1 protein de novo in transgenic plants. Taken together, our results suggest that under excess light conditions, GR2 plays an important role in maintaining both the function of the acceptor side of PSII and the repair of photodamaged PSII by preventing the accumulation of H2O2. In addition, our results provide details of the role of H2O2 in vivo accumulation in photoinhibition in plants. (C) 2016 Elsevier B.V. All rights reserved.
学科主题	Biochemistry & Molecular Biology ; Biophysics
电子版国际标准刊号	0006-3002
出版地	AMSTERDAM
WOS关键词	LOW-TEMPERATURE PHOTOINHIBITION ; TRANSGENIC NICOTIANA-TABACUM ; BACK ELECTRON FLOW ; HYDROGEN-PEROXIDE ; D1 PROTEIN ; CHLOROPHYLL-FLUORESCENCE ; SINGLET OXYGEN ; OXIDATIVE STRESS ; PHOTOSYNTHETIC MACHINERY ; MEMBRANE-LIPIDS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000376700000005
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30970218] ; State Key Basic Research and Development Plan of ChinaNational Basic Research Program of China [2015CB150105]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25312]
专题	中科院光生物学重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Bot, Photosynth Res Ctr, Key Lab Photobiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Ding, Shunhua,Jiang, Rui,Lu, Qingtao,et al. Glutathione reductase 2 maintains the function of photosystem II in Arabidopsis under excess light[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,2016,1857(6):665-677.
APA	Ding, Shunhua,Jiang, Rui,Lu, Qingtao,Wen, Xiaogang,&Lu, Congming.(2016).Glutathione reductase 2 maintains the function of photosystem II in Arabidopsis under excess light.BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,1857(6),665-677.
MLA	Ding, Shunhua,et al."Glutathione reductase 2 maintains the function of photosystem II in Arabidopsis under excess light".BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS 1857.6(2016):665-677.