The Structurally Plastic CH2 Domain Is Linked to Distinct Functions of Fimbrins/Plastins

doi:10.1074/jbc.M116.730069

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	The Structurally Plastic CH2 Domain Is Linked to Distinct Functions of Fimbrins/Plastins
	Zhang, Ruihui 2; Chang, Ming 1; Zhang, Meng 2; Wu, Youjun 5; Qu, Xiaolu 1,3; Huang, Shanjin 1
刊名	JOURNAL OF BIOLOGICAL CHEMISTRY
	2016
卷号	291 期号:34 页码:17881-17896
关键词	actin Arabidopsis Arp2 3 complex cytoskeleton microfilaments plant
DOI	10.1074/jbc.M116.730069
文献子类	Article
英文摘要	Fimbrins/plastins have been implicated in the generation of distinct actin structures, which are linked to different cellular processes. Historically, fimbrins/plastins were mainly considered as generating tight actin bundles. Here, we demonstrate that different members of the fimbrin/plastin family have diverged biochemically during evolution to generate either tight actin bundles or loose networks with distinct biochemical and biophysical properties. Using the phylogenetically and functionally distinct Arabidopsis fimbrins FIM4 and FIM5 we found that FIM4 generates both actin bundles and cross-linked actin filaments, whereas FIM5 only generates actin bundles. The distinct functions of FIM4 and FIM5 are clearly observed at single-filament resolution. Domain swapping experiments showed that cooperation between the conformationally plastic calponin-homology domain 2 (CH2) and the N-terminal headpiece determines the function of the full-length protein. Our study suggests that the structural plasticity of fimbrins/plastins has biologically meaningful consequences, and provides novel insights into the structure-function relationship of fimbrins/plastins as well as shedding light on how cells generate distinct actin structures.
学科主题	Biochemistry & Molecular Biology
电子版国际标准刊号	1083-351X
出版地	BETHESDA
WOS关键词	POLLEN-TUBE GROWTH ; ACTIN-BINDING-PROTEINS ; CALPONIN HOMOLOGY DOMAINS ; ALPHA-ACTININ ; F-ACTIN ; ARP2/3 COMPLEX ; MUSCULAR-DYSTROPHY ; MISSENSE MUTATIONS ; FISSION YEAST ; HUMAN FIMBRIN
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
WOS记录号	WOS:000383241300032
资助机构	Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2013CB945100] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31125004] ; Tsinghua-Peking Joint Center for Life Sciences ; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2015M571028]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25348]
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Tsinghua Peking Joint Ctr Life Sci, Beijing 100084, Peoples R China 3.Tsinghua Univ, Sch Life Sci, Ctr Plant Biol, Beijing 100084, Peoples R China 4.Dartmouth Coll, Dept Biol Sci, Hanover, NH 03755 USA 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Ruihui,Chang, Ming,Zhang, Meng,et al. The Structurally Plastic CH2 Domain Is Linked to Distinct Functions of Fimbrins/Plastins[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2016,291(34):17881-17896.
APA	Zhang, Ruihui,Chang, Ming,Zhang, Meng,Wu, Youjun,Qu, Xiaolu,&Huang, Shanjin.(2016).The Structurally Plastic CH2 Domain Is Linked to Distinct Functions of Fimbrins/Plastins.JOURNAL OF BIOLOGICAL CHEMISTRY,291(34),17881-17896.
MLA	Zhang, Ruihui,et al."The Structurally Plastic CH2 Domain Is Linked to Distinct Functions of Fimbrins/Plastins".JOURNAL OF BIOLOGICAL CHEMISTRY 291.34(2016):17881-17896.