Fast identification of the antibacterial in Aspergillus species

doi:10.18054/pb.v118i4.4155

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	Fast identification of the antibacterial in Aspergillus species
	Wang, Xiaojing ; Duan, Lixin 3; Yang, Shengnan ; Zhou, Zhengfu ; Dun, Baoqing 1
刊名	PERIODICUM BIOLOGORUM
	2016
卷号	118 期号:4 页码:425-430
关键词	Antibacterial activity HPLC Aspergillus Bioactive metabolites
ISSN号	0031-5362
DOI	10.18054/pb.v118i4.4155
文献子类	Article
英文摘要	Background and Purpose: Aspergillus genus biosynthesize a large number of metabolites with wide-ranging bioactivities, but how to target the active compounds quickly and accurately? In this work, we provide a strategy to fast identify bioactive metabolites in fungi with similar morphology but different antimicrobial activities. Materials and Methods: Combined with High Performance Liquid Chromatography (HPLC) profiles and bioactivity screening of fungal extracts, three Aspergillus sp. strains YSN038, YSN052 and YSN064 were studied. The bacteria Bacillus pumilus, Bacillus subtilis, Staphylococcus aureus, Kocuria rhizophila, Escherichia coli and Ralstonia solanacearum were used for antibiotic assay. Results: Three strains showed the similar morphology, but the crude extracts of YSN038 and YSN064 displayed antibacterial activities against B. pumilus, B. subtilis, S. aureus and K. rhizophila, whereas YSN052 had not. The compound contributed to antibacterial activity in vitro was rapidly identified, isolated and characterized. Compound 3 connected with specific HPLC peak should be the antibiotic substance by analysis of HPLC profiles, and was confirmed after the following antimicrobial tests. The compound was elucidated as butyrolactone I based on nuclear magnetic resonance(NMR) and mass spectrometry (MS) data, and showed various biological activities. Conclusions: Our study has significant scope on targeting antibacterial metabolites. The described method could also be used as a rapid and cost-effective tool for screening other bioactivity products. As such, this article could offer a fast approach to isolate drug-lead compounds from microorganisms.
学科主题	Biology
出版地	ZAGREB
WOS关键词	BUTYROLACTONE-I DERIVATIVES ; BOVINE OOCYTES ; TERREUS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	PERIODICUM BIOLOGORUM
WOS记录号	WOS:000397425700013
资助机构	National Basic Research (973) Program of ChinaNational Basic Research Program of China [2015CB755703] ; 948 program of Ministry of Agriculture of China [2016-X43]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/24954]
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 2.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China 3.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xiaojing,Duan, Lixin,Yang, Shengnan,et al. Fast identification of the antibacterial in Aspergillus species[J]. PERIODICUM BIOLOGORUM,2016,118(4):425-430.
APA	Wang, Xiaojing,Duan, Lixin,Yang, Shengnan,Zhou, Zhengfu,&Dun, Baoqing.(2016).Fast identification of the antibacterial in Aspergillus species.PERIODICUM BIOLOGORUM,118(4),425-430.
MLA	Wang, Xiaojing,et al."Fast identification of the antibacterial in Aspergillus species".PERIODICUM BIOLOGORUM 118.4(2016):425-430.