Regio-selective Reduction of Oxysophoridine by Microorganism Isolated from Soil

doi:10.1002/jccs.201500501

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	Regio-selective Reduction of Oxysophoridine by Microorganism Isolated from Soil
	Fu, Shaobin ; Li, Yi ; Meng, Qingfeng 5; Yue, Changwu 1; Sun, Yongzhen 4; Wei, Gang 3
刊名	JOURNAL OF THE CHINESE CHEMICAL SOCIETY
	2016
卷号	63 期号:4
关键词	Biocatalysis Region-stereoselectivity Soil microbes Natural product Condition optimization
ISSN号	0009-4536
DOI	10.1002/jccs.201500501
文献子类	Article
英文摘要	Oxysophoridine (OSR), an alkaloid extracted from a traditional Chinese medicine, attracted more and more attention because of remarkable biological activities. Biocatalysis of OSR by 86 stains including soil microbes and endophytic fungi were investigated. Incubation of OSR with soil bacteria T003 identified as Escherichia coli could lead to only one reductive product with highly regio-selectivity. The structural elucidations of transformation product were achieved mainly by the NMR spectroscopic methods and references. Effect of pH, substrate concentration, conversion time and strain concentration was studied, the optimization of reaction was analyzed by LC-MS. The production of sophoridine (SR) from OSR was highest at pH 7.0, 36 h of incubation and in presence of 625 mu g/mL substrate concentration, 50 mu L/mL strain concentration respectively. Biocatalysis was considered the alternatives to preparation of sophoridine with green and sustainable synthetic processes except for less time-consuming and more environmentally friendly.
学科主题	Chemistry, Multidisciplinary
电子版国际标准刊号	2192-6549
出版地	WEINHEIM
WOS关键词	MICE ; EXPRESSION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000375554200003
资助机构	National Natural Sciences Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21462057] ; Program of Ministry of Education Chunhui Plan [Z2014091] ; Guizhou Science and Technology Department [QKHLH-2014-7555] ; Program for Guizhou Key Laboratory of Microbial Resources & Drug Development [GZMRD-2014-003] ; Ntional Training Program of Innovation and Entrepreneurship for Undergraduates [201410661013]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/24328]
专题	中科院植物分子生理学重点实验室
作者单位	1.Zunyi Med Coll, Dept Publ Hlth, Zunyi 563000, Peoples R China 2.CSIRO, Mat Sci & Engn, Sydney 2070, NSW, Australia 3.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 4.Zunyi Med Coll, Guizhou Key Lab Microbial Resources & Drug Dev, Zunyi 563003, Peoples R China 5.Zunyi Med Coll, Dept Pharm, Zunyi 563000, Peoples R China
推荐引用方式 GB/T 7714	Fu, Shaobin,Li, Yi,Meng, Qingfeng,et al. Regio-selective Reduction of Oxysophoridine by Microorganism Isolated from Soil[J]. JOURNAL OF THE CHINESE CHEMICAL SOCIETY,2016,63(4).
APA	Fu, Shaobin,Li, Yi,Meng, Qingfeng,Yue, Changwu,Sun, Yongzhen,&Wei, Gang.(2016).Regio-selective Reduction of Oxysophoridine by Microorganism Isolated from Soil.JOURNAL OF THE CHINESE CHEMICAL SOCIETY,63(4).
MLA	Fu, Shaobin,et al."Regio-selective Reduction of Oxysophoridine by Microorganism Isolated from Soil".JOURNAL OF THE CHINESE CHEMICAL SOCIETY 63.4(2016).