Fluorescein-Based Chromogenic and Ratiometric Fluorescence Probe for Highly Selective Detection of Cysteine and Its Application in Bioimaging

doi:10.1021/acs.analchem.6b04431

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	Fluorescein-Based Chromogenic and Ratiometric Fluorescence Probe for Highly Selective Detection of Cysteine and Its Application in Bioimaging
	Fu, Zhen-Hai; Han, Xiao; Shao, Yongliang; Fang, Jianguo; Zhang, Zhi-Hong; Wang, Ya-Wen; Peng, Yu
刊名	ANALYTICAL CHEMISTRY
	2017-02-07
卷号	89 期号:3 页码:1937-1944
ISSN号	0003-2700
DOI	10.1021/acs.analchem.6b04431
英文摘要	A dual mode fluorescent probe, which is based on an integration of fluorescein and coumarin fluorophores, was developed for the discrimination of Cys from Hcy and GSH. This probe (2) shows the advantage of quick reaction (5 min) with Cys, resulting in a strong fluorescence turn-on response when excited at 450 nm. Notably, it also demonstrates the ratiometric fluorescence property while excited by a shorter wavelength (332 nm). All of results suggest probe 2 has a high selectivity toward Cys even in the presence of other amino acids, cations and anions. The detection limit of Cys was calculated as 0.084 mu M, which was much lower than the intracellular concentration. H-1 NMR, MS and DFT calculation were used to reveal the detection mechanism further. Finally, this low cytotoxic probe was successfully applied in bioimaging within HepG2 cells.
内容类型	期刊论文
源URL	[http://ir.isl.ac.cn/handle/363002/34521]
专题	青海盐湖研究所_盐湖资源与化学实验室
推荐引用方式 GB/T 7714	Fu, Zhen-Hai,Han, Xiao,Shao, Yongliang,et al. Fluorescein-Based Chromogenic and Ratiometric Fluorescence Probe for Highly Selective Detection of Cysteine and Its Application in Bioimaging[J]. ANALYTICAL CHEMISTRY,2017,89(3):1937-1944.
APA	Fu, Zhen-Hai.,Han, Xiao.,Shao, Yongliang.,Fang, Jianguo.,Zhang, Zhi-Hong.,...&Peng, Yu.(2017).Fluorescein-Based Chromogenic and Ratiometric Fluorescence Probe for Highly Selective Detection of Cysteine and Its Application in Bioimaging.ANALYTICAL CHEMISTRY,89(3),1937-1944.
MLA	Fu, Zhen-Hai,et al."Fluorescein-Based Chromogenic and Ratiometric Fluorescence Probe for Highly Selective Detection of Cysteine and Its Application in Bioimaging".ANALYTICAL CHEMISTRY 89.3(2017):1937-1944.