Drug-Induced Changes of Topography and Elasticity in Living B Lymphoma Cells Based on Atomic Force Microscopy | |
Li M(李密); Liu LQ(刘连庆); Wang YC(王越超); Dong ZL(董再励); Xiao XB(肖秀斌); Zhang WJ(张伟京) | |
刊名 | 物理化学学报
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2012 | |
卷号 | 28期号:6页码:1502-1508 |
关键词 | Atomic Force Microscopy Lymphoma Elasticity Force Curve Youngʹs Modulus |
ISSN号 | 1000-6818 |
其他题名 | 基于AFM的药物刺激前后淋巴瘤活细胞的形貌及弹性的变化 |
产权排序 | 1 |
英文摘要 | Atomic force microscopy(AFM) provides a means for characterizing the surface topography and biophysical properties of individual living cells under near-physiological conditions.However,owing to the lack of adequate cellular immobilization methods,AFM imaging of living,suspended mammalian cells is still a big challenge.In this paper,a method is presented for immobilizing individual living B lymphoma cells that combines mechanical trapping with pillar arrays and electrostatic adsorption with poly-L-lysine.In this way,the topography and elasticity changes of individual B lymphoma cells that were stimulated with different concentrations of Rituximab were observed and measured dynamically.When the cell is stimulated by 0.2 mg·mL-1Rituximab for 2 h,the cell topography becomes more corrugated and Young smodulus decreases from 196 to 183 kPa.When the cell is stimulated by 0.5 mg·mL-1Rituximab for 2 h,thecell topography changes more significantly and some tubercles appear,and Young s modulus decreasesfrom 234 to 175 kPa.These results thus provide a unique insight into the effects of Rituximab on individualcells. |
WOS关键词 | FIBROBLASTS ; INHIBITION |
WOS研究方向 | Chemistry |
语种 | 英语 |
CSCD记录号 | CSCD:4546448 |
WOS记录号 | WOS:000305035700031 |
资助机构 | The project was supported by the National Natural Science Foundation of China(60904095,61175103) ; National High Technology Research and Development Program of China(863)(2009AA03Z316) ; Chinese Academy of Sciences Foreign Experts Affairs International Partnership Program for Creative Research Teams |
公开日期 | 2012-10-24 |
内容类型 | 期刊论文 |
源URL | [http://ir.sia.cn/handle/173321/9989] ![]() |
专题 | 沈阳自动化研究所_机器人学研究室 |
通讯作者 | Liu LQ(刘连庆); Wang YC(王越超) |
作者单位 | 1.Graduate University of Chinese Academy of Sciences, Beijing 100049, P. R. China 2.Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing 100071, P. R. China 3.Department of Electrical and Computer Engineering, Michigan State University, East Lansing 48824, USA 4.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, P. R. China |
推荐引用方式 GB/T 7714 | Li M,Liu LQ,Wang YC,et al. Drug-Induced Changes of Topography and Elasticity in Living B Lymphoma Cells Based on Atomic Force Microscopy[J]. 物理化学学报,2012,28(6):1502-1508. |
APA | Li M,Liu LQ,Wang YC,Dong ZL,Xiao XB,&Zhang WJ.(2012).Drug-Induced Changes of Topography and Elasticity in Living B Lymphoma Cells Based on Atomic Force Microscopy.物理化学学报,28(6),1502-1508. |
MLA | Li M,et al."Drug-Induced Changes of Topography and Elasticity in Living B Lymphoma Cells Based on Atomic Force Microscopy".物理化学学报 28.6(2012):1502-1508. |
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