Hydroxypropyltrimethyl ammonium chloride chitosan-based hydrogel as the split H5N1 mucosal adjuvant: Structure-activity relationship

doi:10.1016/j.carbpol.2021.118139

	Hydroxypropyltrimethyl ammonium chloride chitosan-based hydrogel as the split H5N1 mucosal adjuvant: Structure-activity relationship
	Fan, Qingze 1,3; Miao, Chunyu 3; Huang, Yilan 1; Yue, Hua 3; Wu, Anguo 2; Wu, Jianming 2; Wu, Jie 3; Ma, Guanghui 3
刊名	CARBOHYDRATE POLYMERS
	2021-08-15
卷号	266 页码:10
关键词	Chitosan-based hydrogel Structure activity relationship Intranasal immunization Adjuvant
ISSN号	0144-8617
DOI	10.1016/j.carbpol.2021.118139
英文摘要	In this study, 2-hydroxypropyltrimethyl ammonium chloride chitosan (HTCC)-based hydrogel was devised as a mucosal adjuvant for H5N1 vaccine. Aimed to investigate the structure activity relationship between HTCC hydrogel and immune response, we prepared a series of HTCC hydrogel with defined quaternization degrees (DQs, 0%, 21%, 41%, 60%, 80%). Results suggested that with DQ increasing, the positive charge and gelation time of HTCC hydrogel increased but the viscosity decreased. We applied in vivo imaging system and found that the moderate DQ 41% prolonged antigen residence time in nasal cavity, resulting in the most potent systemic responses (IgG, IgG1, IgG2a, HI). While, the lowest DQ 0% produced the best mucosal IgA antibody responses, most likely due to the closer contact with mucosa. Furthermore, the influence of animal gender was also discussed. These data add to the growing understanding of the relationship between physicochemical features of chitosan-based hydrogel and how they influence the immune responses.
资助项目	National Key Research & Development Program of China[2018YFC0311303] ; National Natural Science Foundation of China (NSFC)[21776287] ; National Natural Science Foundation of China (NSFC)[22078335] ; Luzhou Science and Technology Program[2018-JYJ-41] ; Foundation of Southwest Medical University[2017-ZRZD-003] ; Southwest Medical University[2017-LH011] ; Southwest Medical University[2018LZXNYD-PT02] ; Ph.D. Research Startup Foundation of the Affiliated Hospital of Southwest Medical University[17136] ; Collaborative Project of Luzhou TCM Hospital[2017-LH011] ; Collaborative Project of Luzhou Government[2018LZXNYD-PT02]
WOS关键词	N-TRIMETHYL CHITOSAN ; IMMUNE-RESPONSE ; CLEARANCE CHARACTERISTICS ; QUATERNIZED CHITOSAN ; VACCINE ; VIRUS ; MICE ; TMC ; IMMUNOGENICITY ; TRANSMISSION
WOS研究方向	Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000655700400006
资助机构	National Key Research & Development Program of China ; National Natural Science Foundation of China (NSFC) ; Luzhou Science and Technology Program ; Foundation of Southwest Medical University ; Southwest Medical University ; Ph.D. Research Startup Foundation of the Affiliated Hospital of Southwest Medical University ; Collaborative Project of Luzhou TCM Hospital ; Collaborative Project of Luzhou Government
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/49033]
专题	中国科学院过程工程研究所
通讯作者	Wu, Jie; Ma, Guanghui
作者单位	1.Southwest Med Univ, Dept Pharm, Affiliated Hosp, Luzhou 646000, Sichuan, Peoples R China 2.Southwest Med Univ, Sch Pharm, Dept Pharmacol, Luzhou 646000, Sichuan, Peoples R China 3.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, 1 Bei Er Jie, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Fan, Qingze,Miao, Chunyu,Huang, Yilan,et al. Hydroxypropyltrimethyl ammonium chloride chitosan-based hydrogel as the split H5N1 mucosal adjuvant: Structure-activity relationship[J]. CARBOHYDRATE POLYMERS,2021,266:10.
APA	Fan, Qingze.,Miao, Chunyu.,Huang, Yilan.,Yue, Hua.,Wu, Anguo.,...&Ma, Guanghui.(2021).Hydroxypropyltrimethyl ammonium chloride chitosan-based hydrogel as the split H5N1 mucosal adjuvant: Structure-activity relationship.CARBOHYDRATE POLYMERS,266,10.
MLA	Fan, Qingze,et al."Hydroxypropyltrimethyl ammonium chloride chitosan-based hydrogel as the split H5N1 mucosal adjuvant: Structure-activity relationship".CARBOHYDRATE POLYMERS 266(2021):10.