Rapid and high-capacity loading of IgG monoclonal antibodies by polymer brush and peptides functionalized microspheres

doi:10.1016/j.chroma.2021.461948

	Rapid and high-capacity loading of IgG monoclonal antibodies by polymer brush and peptides functionalized microspheres
	Hao, Dongxia 1; Zhang, Rongyue 2; Ge, Jia 1; Ye, Peili 1; Song, Cui 1; Zhu, Kai 1; Na, Xiangming 1; Huang, Yongdong 1; Zhao, Lan 1; Zhou, Weiqing 1
刊名	JOURNAL OF CHROMATOGRAPHY A
	2021-03-15
卷号	1640 页码:10
关键词	Antibody purification Peptide ligand Polymer brush Macroporous microspheres
ISSN号	0021-9673
DOI	10.1016/j.chroma.2021.461948
英文摘要	Fast-throughput and cost reduction of current purification platforms are becoming increasing requests during antibody manufacture. The macroporous-matrix absorbents have presented extensive potentiality in improving operational throughput during purification of macromolecule. And meanwhile the peptide ligand has become a promising alternative to recombinant protein ligands for cost reduction of chromatographic purification. Therefore, here we designed a functionalized microspheres resin with both macroporous matrix of polymerized glycidyl methacrylate and ethylene glycol dimethacrylate (PGMA-EDMA) and peptide ligand of hexapeptide (FYEILH). In order to circumvent the steric effect of peptides and amplify the binding sites on macroporous matrix, the peptide ligand was coupled on a liner PGMA polymer brushes grafted on microspheres. Comparing to the conventional agarose-matrix resin and the general peptide-grafted microspheres, the functionalized microspheres presented excellent permeability and high capacity to rapid loading hIgG by maintaining a stable level of dynamic binding capacity at fast flow rate above 110 column volume per hour (cv/h) and very short residence time below 0.5 min. Such functionalized microspheres provide a facile and broadly applicable strategy to develop the attractive candidate for rapid and cost-reduced purification of antibody. (c) 2021 Elsevier B.V. All rights reserved.
资助项目	National Natural Science Foundation of China[21676275] ; National Natural Science Foundation of China[22078334] ; National Natural Science Foundation of China[21821005] ; Beijing Natural Science Foundation[L160012] ; Youth Top Talent Project of Organization Department of Beijing Municipal Committee[ZZB2019001] ; National Key Research and Development Program of China[2018YFC0311101] ; Beijing Natural Science FoundationHaidian Original Innovation Joint Fund[L192017]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000624599200011
资助机构	National Natural Science Foundation of China ; Beijing Natural Science Foundation ; Youth Top Talent Project of Organization Department of Beijing Municipal Committee ; National Key Research and Development Program of China ; Beijing Natural Science FoundationHaidian Original Innovation Joint Fund
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/48071]
专题	中国科学院过程工程研究所
通讯作者	Ma, Guanghui
作者单位	1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, POB 353, Beijing 100190, Peoples R China 2.Beijing Inst Petrochem Technol, Beijing 102617, Peoples R China
推荐引用方式 GB/T 7714	Hao, Dongxia,Zhang, Rongyue,Ge, Jia,et al. Rapid and high-capacity loading of IgG monoclonal antibodies by polymer brush and peptides functionalized microspheres[J]. JOURNAL OF CHROMATOGRAPHY A,2021,1640:10.
APA	Hao, Dongxia.,Zhang, Rongyue.,Ge, Jia.,Ye, Peili.,Song, Cui.,...&Ma, Guanghui.(2021).Rapid and high-capacity loading of IgG monoclonal antibodies by polymer brush and peptides functionalized microspheres.JOURNAL OF CHROMATOGRAPHY A,1640,10.
MLA	Hao, Dongxia,et al."Rapid and high-capacity loading of IgG monoclonal antibodies by polymer brush and peptides functionalized microspheres".JOURNAL OF CHROMATOGRAPHY A 1640(2021):10.