Evaluation of inhibitory effects of geniposide on a tumor model of human breast cancer based on 3D printed Cs/Gel hybrid scaffold

doi:10.1016/j.msec.2020.111509

	Evaluation of inhibitory effects of geniposide on a tumor model of human breast cancer based on 3D printed Cs/Gel hybrid scaffold
	Lv, Kena 1; Zhu, Jingjing 1; Zheng, Shuangshuang 2; Jiao, Zeren 3; Nie, Yi 2,4; Song, Fei 5; Liu, Tianqing 1; Song, Kedong 1
刊名	MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
	2021-02-01
卷号	119 页码:10
关键词	Geniposide Anti-tumor MCF-7 cells 3D Cs/Gel scaffold
ISSN号	0928-4931
DOI	10.1016/j.msec.2020.111509
英文摘要	Traditional Chinese medicine therapy, which can serve as adjuvant therapy for cancer treatment, has no obvious side effects on the human body. Geniposide (GEN), one of the main iridoid glycosides in gardenia fruit, has been widely reported to have anti-cancer effects. In this study, we aimed to inspect whether GEN could inhibit proliferation and promote the apoptosis of human breast cancer cells (MCF-7). In order to better predict the efficacy of GEN, we have prepared the Cs/Gel composite scaffolds by 3D printing technology to mimic the MCF-7 cell growth microenvironment. The prepared Cs/Gel scaffold has good mechanical properties and biocompatibility, which can provide a more accurate platform for drug screening. The semi-inhibitory concentration (IC50) evaluated by CCK-8 assay was 16.06 mg/mL (24 h), 14.85 mg/mL (48 h), and 13.14 mg/mL (72 h). After exposed to GEN for 48 h, the cancer cell survival rate reduced from 69.15 +/- 2.86% (13 mg/mL) to 20.97 +/- 3.24% (16 mg/mL). Although the inhibitory effect was weaker in the 3D culture system, it also managed to inhibit cell proliferation and induce cell apoptosis. Besides, Live/Dead staining, Hematoxylin-Eosin (H&E) staining and SEM evaluation were also conducted to estimate the anti-cancer effect of GEN in 2D and 3D cultures. The results indicate that GEN has an anti-cancer effect based on a timeand dose-dependent manner.
资助项目	National Natural Science Foundation of China[31670978/31370991/21676041] ; Fok Ying Tung Education Foundation[132027] ; State Key Laboratory of Fine Chemicals[KF1111] ; Natural Science Foundation of Liaoning Province[20180510028] ; Fundamental Research Funds for the Central Universities[DUT20ZD206/DUT20YG118]
WOS关键词	ASTRAGALUS POLYSACCHARIDES ; INDUCED APOPTOSIS ; CULTURE ; CELLS ; ARCHITECTURE ; EXPRESSION
WOS研究方向	Materials Science
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000600891400004
资助机构	National Natural Science Foundation of China ; Fok Ying Tung Education Foundation ; State Key Laboratory of Fine Chemicals ; Natural Science Foundation of Liaoning Province ; Fundamental Research Funds for the Central Universities
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/42982]
专题	中国科学院过程工程研究所
通讯作者	Nie, Yi; Song, Fei; Liu, Tianqing; Song, Kedong
作者单位	1.Dalian Univ Technol, State Key Lab Fine Chem, Dalian R&D Ctr Stem Cell & Tissue Engn, Dalian 116024, Peoples R China 2.Zhengzhou Inst Emerging Ind Technol, Zhengzhou 450000, Peoples R China 3.Texas A&M Univ, Artie McFerrin Dept Chem Engn, College Stn, TX 77843 USA 4.Chinese Acad Sci, Key Lab Green Proc & Engn, Inst Proc Engn, Beijing 100190, Peoples R China 5.Dalian Med Univ, Hosp 2, Dept Neurosurg, Dalian 116027, Peoples R China
推荐引用方式 GB/T 7714	Lv, Kena,Zhu, Jingjing,Zheng, Shuangshuang,et al. Evaluation of inhibitory effects of geniposide on a tumor model of human breast cancer based on 3D printed Cs/Gel hybrid scaffold[J]. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,2021,119:10.
APA	Lv, Kena.,Zhu, Jingjing.,Zheng, Shuangshuang.,Jiao, Zeren.,Nie, Yi.,...&Song, Kedong.(2021).Evaluation of inhibitory effects of geniposide on a tumor model of human breast cancer based on 3D printed Cs/Gel hybrid scaffold.MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,119,10.
MLA	Lv, Kena,et al."Evaluation of inhibitory effects of geniposide on a tumor model of human breast cancer based on 3D printed Cs/Gel hybrid scaffold".MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS 119(2021):10.