Liquid-Phase and Ultrahigh-Frequency-Acoustofluidics-Based Solid-Phase Synthesis of Biotin-Tagged 6 '/3 '-Sialyl-N-Acetylglucosamine by Sequential One-Pot Multienzyme System

doi:10.3390/catal10111347

	Liquid-Phase and Ultrahigh-Frequency-Acoustofluidics-Based Solid-Phase Synthesis of Biotin-Tagged 6 '/3 '-Sialyl-N-Acetylglucosamine by Sequential One-Pot Multienzyme System
	Gong, Mengge 2,4; Li, Tiechuan 1; Wu, Lina 2; Zhang, Zhenxing 5; Ren, Lishi 2; Duan, Xuexin ; Cao, Hongzhi 3; Pei, Meishan 4; Li, Jian-Jun 2; Du, Yuguang 2
刊名	CATALYSTS
	2020-11-01
卷号	10 期号:11 页码:12
关键词	biotinylated 6 '/3 '-sialyl-N-acetylglucosamine one-pot multienzyme solid-phase oligosaccharide synthesis influenza viruses ultrahigh-frequency-acoustofluidics
DOI	10.3390/catal10111347
英文摘要	6 '/3 '-Sialylated N-acetyllactosamine (6 '/3 '-SLN) is important for discrimination of the source (human or avian) of influenza virus strains. Biotinylated oligosaccharides have been widely used for analysis and quick detection. The development of efficient strategies to synthesize biotin-tagged 6 '/3 '-SLN have become necessary. Effective mixing is essential for enzymatic solid-phase oligosaccharide synthesis (SPOS). In the current study, newly developed technology ultrahigh-frequency-acoustofluidics (UHFA), which can provide a powerful source for efficient microfluidic mixing, solid-phase oligosaccharide synthesis and one-pot multienzyme (OPME) system, were used to develop a new strategy for oligosaccharide synthesis. Firstly, biotinylated N-acetylglucosamine was designed and chemically synthesized through traditional approaches. Secondly, biotinylated 6 '- and 3 '-sialyl-N-acetylglucosamines were prepared in solution through two sequential OPME modules in with a yield of similar to 95%. Thirdly, 6 '-SLN was also prepared through UHFA-based enzymatic solid-phase synthesis on magnetic beads with a yield of 64.4%. The current strategy would be potentially used for synthesis of functional oligosaccharides.
资助项目	National Natural Science Foundation of China[21877114]
WOS关键词	EFFICIENT CHEMOENZYMATIC SYNTHESIS ; ACID ; OLIGOSACCHARIDE ; TETRASACCHARIDE ; STREPTAVIDIN ; AFFINITY ; GLYCANS ; CANCER ; ROLES ; CELLS
WOS研究方向	Chemistry
语种	英语
出版者	MDPI
WOS记录号	WOS:000593655800001
资助机构	National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/42729]
专题	中国科学院过程工程研究所
通讯作者	Pei, Meishan; Li, Jian-Jun
作者单位	1.Tianjin Univ, State Key Lab Precis Measuring Technol & Instrume, Tianjin 300072, Peoples R China 2.Chinese Acad Sci, Natl Key Lab Biochem Engn, Natl Engn Res Ctr Biotechnol Beijing, PLA,Inst Proc Engn,Key Lab Biopharmaceut Prod & F, Beijing 100190, Peoples R China 3.Shandong Univ, State Key Lab Microbial Technol, Natl Glycoengn Res Ctr, Qingdao 266237, Peoples R China 4.Univ Jinan, Sch Chem & Chem Engn, Jinan 250022, Peoples R China 5.Chinese Acad Sci, Inst Microbiol, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Gong, Mengge,Li, Tiechuan,Wu, Lina,et al. Liquid-Phase and Ultrahigh-Frequency-Acoustofluidics-Based Solid-Phase Synthesis of Biotin-Tagged 6 '/3 '-Sialyl-N-Acetylglucosamine by Sequential One-Pot Multienzyme System[J]. CATALYSTS,2020,10(11):12.
APA	Gong, Mengge.,Li, Tiechuan.,Wu, Lina.,Zhang, Zhenxing.,Ren, Lishi.,...&Du, Yuguang.(2020).Liquid-Phase and Ultrahigh-Frequency-Acoustofluidics-Based Solid-Phase Synthesis of Biotin-Tagged 6 '/3 '-Sialyl-N-Acetylglucosamine by Sequential One-Pot Multienzyme System.CATALYSTS,10(11),12.
MLA	Gong, Mengge,et al."Liquid-Phase and Ultrahigh-Frequency-Acoustofluidics-Based Solid-Phase Synthesis of Biotin-Tagged 6 '/3 '-Sialyl-N-Acetylglucosamine by Sequential One-Pot Multienzyme System".CATALYSTS 10.11(2020):12.