Thermolysin-triggered short peptides self-assembly in confined space and application in cell culturing

doi:10.1016/j.colsurfa.2020.125213

	Thermolysin-triggered short peptides self-assembly in confined space and application in cell culturing
	Wang, Meiyue 1,2; Wang, Anhe 1,2; Li, Jieling 1,2; Li, Qi 1,2; Bai, Shuo 1,2
刊名	COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
	2020-10-20
卷号	603 页码:8
关键词	Self-assembly Short peptide Thermolysin Confined space AAO templates
ISSN号	0927-7757
DOI	10.1016/j.colsurfa.2020.125213
英文摘要	Molecules self-assembly in confined space such as in cell to form microfilaments is a crucial process to achieve special functionality. Herein, short peptides with different sequences were taken as examples to explore their self-assembly behavior in AAO templates which offer different pore size and depth to mimic confined environment. Thermolysin, as a mild, selective and effective biological enzyme, is applied here to trigger the self-assembly of short peptides in the pores of AAO template. Experimental results indicate that the morphology, structure and orientation of short peptide fibers in the pores are significantly affected by pore size of AAO templates. Moreover, the formed short peptide fibers in pores show obvious influence on the cell morphology and spreading. The method proposed here gives a feasible avenue to reveal the effect of confined environment on self-assembly behavior of short peptide.
资助项目	National Natural Science Foundation of China[21774132,21703253]
WOS关键词	BETA-SHEET ; DISCOVERY
WOS研究方向	Chemistry
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000571001200003
资助机构	National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/42165]
专题	中国科学院过程工程研究所
通讯作者	Wang, Anhe; Bai, Shuo
作者单位	1.Univ Chinese Acad Sci, Sch Chem Engn, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Wang, Meiyue,Wang, Anhe,Li, Jieling,et al. Thermolysin-triggered short peptides self-assembly in confined space and application in cell culturing[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2020,603:8.
APA	Wang, Meiyue,Wang, Anhe,Li, Jieling,Li, Qi,&Bai, Shuo.(2020).Thermolysin-triggered short peptides self-assembly in confined space and application in cell culturing.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,603,8.
MLA	Wang, Meiyue,et al."Thermolysin-triggered short peptides self-assembly in confined space and application in cell culturing".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 603(2020):8.